The effect of supplementation of follicular fluid (FF) at 5.0% concentration in in vitro maturation (IVM) media on in vitro production of buffalo embryos was studied. Oocytes were aspirated from slaughter house derived ovaries and graded. Cultutarable quality oocytes were randomly cultured in IVM media with FF (n = 334) and without FF (n = 228) and subjected to maturation. The maturation rate, ...

Substrate utilization in defined media for two flower spiroplasmas (S. floricola and FS SR-3) and honeybee spiroplasma (HBS AS-576) was investigated. Glucose, fructose, and mannose were utilized by all three spiroplasmas. In addition, HBS (AS-576) could ferment trehalose; FS (SR-3), sucrose; and S. floricola, trehalose, sucrose, and raffinose. The three spiroplasmas varied greatly in growth req...

The study was undertaken to elucidatethe beneficial effects of goat follicular fluid (gFF) added to maturation and culture media on in vitro maturation, fertilization and post-fertilization development of oocytes in Black Bengal goats. Follicular fluid and oocytes were collected from slaughter house goat’s ovaries using the aspiration technique. Media were prepared using TCM-199 supplemented wi...

The present study was carried out to examine the effect of maturation media on male pronucleus formation of pig oocyte matured and fertilized in vitro. Follicular oocytes collected from prepubertal gilts at a local slaughter house were cultured (36 h) in three different media (mTCM-199, Waymouth MB 752/l, and mTLP-PVA), fertilized in vitro, and assessed for nuclear maturation and male pronucleu...

Improvements in in vitro maturation techniques have focused on culture optimization to increase oocyte maturation rates. Specialized culture media, now commercially available, did not perform significantly better than standard IVF culture media for maturation of immature oocytes in our normal IVF cases.

Background: Polyunsaturated fatty acids (PUFAs) have been shown to influence fertility and endocrinology of reproduction and metabolic activity in many species. In dairy cows, we and others have shown changes in steroid and metabolic hormones and prostaglandins leading to alteration of ovarian activity and uterine function. These can influence fertility by changes in folliculogenesis cyclicity ...

In vitro maturation (IVM) is emerging as a popular technology at the forefront of fertility treatment and preservation. However, standard in vitro culture conditions usually increase reactive oxygen species (ROS), which have been implicated as one of the major causes for reduced embryonic developmental competence. It is well-known that higher than physiological levels of ROS triggers granulosa ...

the development of culture media able to mimic the preovulatory stage of follicular environment and support nuclear and cytoplasmic maturation of oocyte is important for in-vitro fertilization (ivf) programs. it seems that the best culture media for embryonic development and cell cleavage is the natural composition which surrounds the oocyte which has been used occasionally in human ivf program...

Background: The maturation medium for in-vitro oocyte maturation is usually supplemented with serum. However, supplementation of serum from pregnant women adversely affects the outcome of in-vitro maturation. The purpose of the study was to assess if growth factors or granulosa cell coculture could overcome the adverse effects of pregnant women’s serum. Materials and Methods: The basal maturati...