Trypsin (E.C.3.4.21.4) is a serine protease commonly used in proteomics for digestion of proteins. In the present study, the effect of nano-TiO2 on the conformation and catalytic activity of trypsin were studied. The thermal denaturation of trypsin has been investigated in the presence and absence of nano-TiO2 over the temperature range (293-373 K) at pH 3.0 and 7.25, using temperature scanning...

A simple and effective device for investigating heat-induced denaturation of proteins by electrospray ionization mass spectrometry is described. Results are presented for the denaturation as a function of temperature and solution pH of bovine ubiquitin and bovine cytochrome c. These results are in concert with and extend the earlier results of LeBlanc et al. (Org. Mass Spectrom. 1991, 26, 831)....

Both sodium chloride and sodium sulfate are able to stabilize yeast frataxin, causing an overall increase of its thermodynamic stability curve, with a decrease in the cold denaturation temperature and an increase in the hot denaturation one. The influence of low concentrations of these two salts on yeast frataxin stability can be assessed by the application of a theoretical model based on scale...

For minor and major DNA alleles, coamplification at lower denaturation temperature-PCR (COLDPCR) selectively enriches minority alleles from mixtures of wild-type and mutation-containing sequences without a priori knowledge of the type or position of a mutation within a sequence (1 ). Since its inception (2 ), COLD-PCR has been employed in a variety of applications, including cancer, prenatal di...

The mechanical properties of tissue engineering scaffolds are critical for preserving the structural integrity and functionality during both in vivo implantation and long-term performance. In addition, the mechanical and structural properties of the scaffold can direct cellular activity within a tissue-engineered construct. In this context, the aim of this study was to investigate the effects o...

The coagulation of proteins has been shown to consist of two distinct processes (2, 3). In the first of these the protein is denatured, and in the second the denatured protein is agglutinated or flocculated. Chick and Martin (3) and more recently Lepeschldn (4) have investigated these two processes separately, and it is to them that we owe much of our knowledge in this field. Most of the work h...

of skin tissue is highly interdisciplinary, involving bioheat transfer, burn damage, biomechanics and physiology. Characterization of the thermomechanical behaviour of skin tissue is of great importance and can contribute to a variety of medical applications. However, few quantitative studies have been conducted on the temperature dependent mechanical properties of skin tissue. This paper repor...

For minor and major DNA alleles, coamplification at lower denaturation temperature-PCR (COLDPCR) selectively enriches minority alleles from mixtures of wild-type and mutation-containing sequences without a priori knowledge of the type or position of a mutation within a sequence (1 ). Since its inception (2 ), COLD-PCR has been employed in a variety of applications, including cancer, prenatal di...

Systematic photometric studies are presented to analyze the thermal denaturation behaviour with and without formamide of metaphase chromosome suspensions in comparison to DNA solutions. Temperature dependent hyperchromicity measurements at 256 nm and 313 nm were performed using an appropriately designed computer-controlled photometer device. Due to an upright optical axis, this allowed absorban...