Effluent from a fish tank with Nile tilapia (Oreochromis niloticus) was treated in system of two parallel fixed-bed anaerobic reactors followed by suspended stirred, anoxic reactor for solids, organic matter and nitrogen removal. Microbial community structure compared between the an enriched anammox culture originated sludge using PCR (Polymerase Chain Reaction) DGGE (Denaturing Gradient Gel El...

results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...

Denaturing high performance liquid chromatography is a relatively new method by which heteroduplex structures formed during the PCR amplification of heterozygote samples can be rapidly identified. The use of this technology for mutation detection in hereditary non-polyposis colorectal cancer (HNPCC) has the potential to appreciably shorten the time it takes to analyze genes associated with this...

the intestinal bacterial diversity of stichopus japonicus was investigated using 16s ribosomal rna gene (rdna) clone library and polymerase chain reaction/denaturing gradient gel electrophoresis (pcr-dgge). the clone library yielded a total of 188 clones, and these were sequenced and classified into 106 operational taxonomic units (otus) with sequence similarity ranging from 88 to 100%. the cov...

The development of methodology to differentiate mixed populations of Escherichia coli in the secondary habitat might improve monitoring of fecal pollution indicators and facilitate the development of strategies to mitigate bacterial pollution. The objective of this study was to determine the ability of denaturing gradient gel electrophoresis (DGGE) to differentiate mixed assemblages of E. coli ...

The availability and application of culture-independent tools that enable a detailed investigation of the microbiota and microbial biodiversity of food systems has had a major impact on food microbiology. This review focuses on the application of DNA-based technologies, such as denaturing gradient gel electrophoresis (DGGE), temporal temperature gradient gel electrophoresis (TTGE), single stran...

The present study was undertaken in a constructed wetland (CW), setup tourism house, for domestic wastewater treatment. influence of season variations on the abundance fecal indicator organisms (total coliforms and Escherichia coli) substrate roots plants inhabiting inlet outlet zones CW evaluated along three consecutive years. structure diversity bacterial communities associated to CW’s also a...

The treatment of cancer depends on the activity cytochrome P450 enzyme family, which is essentially carried out by CYP3A4 and CYP3A5 enzymes. aim our study was to investigate whether polymorphism could contribute protein their influence response cells treatment. variability cDNA profiles between cell lines parental HT-29 resistant HT-29-OxR adenocarcinoma detected using denaturing gradient gel ...

Microbial Enhanced Oil Recovery (MEOR) is an effective alternative method for oil recovery in reservoirs using microorganisms or their secondary metabolites. This research aimed to evaluate the indigenous bacteria from Mae Soon reservoir by culture-dependent and culture-independent methods investigate potential of biosurfactant-producing a drop-collapsed assay. Indigenous were isolated sands re...