نتایج جستجو برای: density gradient centrifugation
تعداد نتایج: 528946 فیلتر نتایج به سال:
OBJECTIVE This study aimed to assess and compare sperm motility, concentration, and morphology recovery rates, before and after processing through sperm washing followed by swim-up or discontinuous density gradient centrifugation in normospermic individuals. METHODS Fifty-eight semen samples were used in double intrauterine insemination procedures; 17 samples (group 1) were prepared with sper...
Background and Objective: Nowadays, mesenchymal stem cells (MSCs) are considered as a promising tool for treatment of different diseases. Due to the low frequency of MSCs, however, it seems inevitable to expand them in vitro prior to use, which could affect the quality of the cells. In all isolation procedures, the density gradient separation of Ficoll is used for volume reduction and RBC exclu...
Using a colloidal silica density gradient, HeLa cells in mitosis were found to have a density of 1.040-1.046 g/cc, lighter than the remaining interphase cells. The mitotic cells could be harvested and cultured after centrifugation, showing growth synchrony by measurement of a peak in mitotic index 21 hr after establishing the culture. By using Colcemid or vinblastine sulfate, HeLa cells were ar...
OBJECTIVE:We evaluated the effectiveness of spermwashing using the discontinuous two-layer density gradient centrifugation method to remove antisperm antibodies attached to the sperm surface. METHOD: We prospectively enrolled sixty-six men with unexplained infertility who were seeking evaluation. Each patient delivered one semen specimen for the study. We determined antisperm antibody levels us...
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