نتایج جستجو برای: dermatophilosis
تعداد نتایج: 67 فیلتر نتایج به سال:
Random peptide libraries (RPL) displayed on the surface of filamentous bacteriophages have been extensively used as a tool to map epitopes or to identify antigenic mimics (mimotpoes) of disease-specific monoclonal antibodies or polyclonal sera. These RPL are engineered by the insertion of degenerate oligonucleotides, encoding a specific number of random amino acids, in frame with a bacteriophag...
A case of dermatophilosis in a horse was described. The disease was diagnosed in a three-year-old mare. Physical examination, cytology, bacterial culture, and histopathological examination were performed. Cytology revealed numerous filamentous chains of bacteria formed into 2 to 4 rows of cells. D. congolensis was grown in a culture. Histopathological examination revealed, pyoderma accompanied ...
random peptide libraries (rpl) displayed on the surface of filamentous bacteriophages have been extensively used as a tool to map epitopes or to identify antigenic mimics (mimotpoes) of disease-specific monoclonal antibodies or polyclonal sera. these rpl are engineered by the insertion of degenerate oligonucleotides, encoding a specific number of random amino acids, in frame with a bacteriophag...
A proliferative dermatitis similar to the condition generally referred to as strawberry footrot was observed in two Alpine chamois (Rupicapra rupicapra) from Eastern Alps, Italy. Branching septated filaments and packets of PAS-positive coccoid organisms were observed in histological sections of the affected skin. The actinomycete, Dermatophilus congolensis, was isolated from crusted lesions in ...
Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis
Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tube...
A partial amino acid sequence of a serine protease from Dermatophilus congolensis allowed the design of oligonucleotide primers that were complemented with additional ones from previously published partial sequences of the gene encoding the enzyme. The polymerase chain reaction (PCR), using combinations of specific and degenerate oligonucleotide primers, allowed the amplification of a 1738-bp i...
Dermatophilus congolensis infection is described for the first time in a woodchuck (Marmota monax) and a striped skunk (Mephitis mephitis). This actinomycete was also recovered from a raccoon (Procyon lotor) carcass which had been frozen for a year. Encrusted lesions or denuded patches or both were seen during necropsy on the head, thorax, abdomen, and legs of each carcass. D. congolensis was d...
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