نتایج جستجو برای: digital microfluidic biochip
تعداد نتایج: 325478 فیلتر نتایج به سال:
An enzyme-containing microfluidic biochip has been developed for the oxidative polymerization of phenols. The biochip consists of a simple T-junction with two feed reservoirs 20 mm apart and a microreaction channel 30 mm long. The channel is 15 microm deep and 200 microm wide at the center, giving a reaction volume of 90 nL. The biochip was fabricated using conventional photolithographic method...
Microfluidic-based biochips are replacing the conventional biochemical analyzers, and are able to integrate all the necessary functions for biochemical analysis. The digital microfluidic biochips are based on the manipulation of liquids not as a continuous flow, but as discrete droplets. Several approaches have been proposed for the synthesis of digital microfluidic biochips, which, starting fr...
A key factor determining the fate of individual cells within an epithelium is the unique microenvironment that surrounds each cell. It regulates location-dependent differentiation into specific cellular sub-types, but, on the other hand, a disturbed microenvironment can promote malignant transformation of epithelial cells leading to cancer formation. Here, we present a tool based on a microflui...
Advances in microfluidic technologies have led to the emergence of biochip devices for automating laboratory procedures in biochemistry and molecular biology. Corresponding systems are revolutionizing a diverse range of applications, e.g. air quality studies, point-of-care clinical diagnostics, drug discovery, and DNA sequencing – with an increasing market. However, this continued growth depend...
Endogenous electric fields (EFs) play an important role in many biological processes. In order to gain an insight into these biological phenomena, externally applied electric fields are used to study cellular responses. In this work, we report the construction and fabrication of a direct current (DC)-electrically stimulated microfluidic biochip and its validation with murine photoreceptor-deriv...
Microfluidic bioartificial organs allow the reproduction of in vivo-like properties such as cell culture in a 3D dynamical micro environment [1]. In this work, we established a method and a protocol for performing the integration of transcriptomic, proteomic and metabolomic profiles in order to investigate xenobiotic toxicity. For that purpose, we present the example of the analysis of HepG2/C3...
Sepsis, a potentially life-threatening complication of an infection, has the highest burden of death and medical expenses in hospitals worldwide. Leukocyte count and CD64 expression on neutrophils (nCD64) are known to correlate strongly with improved sensitivity and specificity of sepsis diagnosis at its onset. A major challenge is the lack of a rapid and accurate point-of-care (PoC) device tha...
Microfluidics-based biochip devices are developed to perform DNA analysis from complex biological sample solutions. Microfluidic mixers, valves, pumps, channels, chambers, heaters, and DNA microarray sensor are integrated to perform magnetic bead-based rare cell capture, cell preconcentration and purification, cell lysis, polymerase chain reaction, DNA hybridization and electrochemical detectio...
Microfluidic-based biochips are replacing the conventional biochemical analyzers, by integrating all the necessary functions for biochemical analysis using microfluidics. The digital microfluidic biochips (DMBs) manipulate discrete amounts of fluids of nanoliter volume, named droplets, on an array of electrodes to perform operations such as dispensing, transport, mixing, split, dilution and det...
Digital microfluidic biochips (DMFBs) are revolutionizing many biochemical analysis procedures, e.g., high-throughput DNA sequencing and point-of-care clinical diagnosis. However, today’s DMFBs suffer from several limitations: (1) constraints on droplet size and the inability to vary droplet volume in a fine-grained manner; (2) the lack of integrated sensors for real-time detection; (3) the nee...
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