Sisal leaves which constitute only 2% of the sisal plant have been used in the production of pulp and fiber; the remaining 98% which is mostly of the sisal bole is largely regarded as waste. The bole’s high inulin fraction (2436%) is of particular significance due to its chemical functionality and relative ease of fermentation. utilisationThe objective of this study was to identify properties a...

To produce chitoorigosaccharides using chitosan, we attempted to construct Paenibacillus fukuinensis chitosanase-displaying yeast cells as a whole-cell biocatalyst through yeast cell-surface engineering. The localization of the chitosanase on the yeast cell surface was confirmed by immunofluorescence labeling of cells. The chitosanase activity of the constructed yeast was investigated by halo a...

BACKGROUND Most of the current colorimetric methods for detection of chitinase or cellulase activities on the insoluble natural polymers chitin and cellulose depend on a chemical redox reaction. The reaction involves the reducing ends of the hydrolytic products. The Schales' procedure and the 3,5-dinitrosalicylic acid (DNS) method are two examples that are commonly used. However, these methods ...

This study investigated lytic enzyme activities in three indigenous Trichoderma strains namely, Trichoderma asperellum, Trichoderma harzianum and Trichoderma sp. Native Trichoderma strains and a virulent strain of Rhizoctonia solani isolated from infected bean plants were also included in the study. Enzyme activities were determined by measuring sugar reduction by dinitrosalicylic acid (DNS) me...

Wenling Medic (Medicago polymorpha) is common in southern China and has long been utilized as a vegetable eastern China, well significant raw ingredient for livestock pickled meals. As legume, there still research vacuum, the first problem faces production of seed germination. The germination percentage was low, according to practice laboratory tests performed this work. Furthermore, after more...

Aim: Response surface methodology (RSM) model was applied to optimize ethanol production from Calabash (Crescentia cujete) pulp juice using Cronobacter malonaticus. Study Design: The squeezed with muslin cloth, and vacuum filtered clear solution before use. tested for reducing sugars the Dinitrosalicylic acid (DNS) method. Twenty three (23) runs, including 3 controls, of fermentation conducted ...