Vaginal discharge specimens from 105 women were examined by wet mount, culture, and a new direct immunofluorescence assay to detect Trichomonas vaginalis. The organism was detected by culture in 31 patients, by direct immunofluorescence in 26 patients, and by wet mount in 21 patients.

Specimens collected from 182 patients with histories suggesting chlamydial disease were examined by conventional culture and direct immunofluorescence techniques. Chlamydia trachomatis was identified by both methods in 57% of all patients. There was no significant difference between the two methods in detecting C trachomatis. Where a tissue culture service is not already established, cost analy...

A direct rapid immunohistochemical test (dRIT) was evaluated under field and laboratory conditions to detect rabies virus antigen in frozen and glycerol-preserved field brain samples from northwestern Tanzania. Compared to the direct fluorescent antibody test, the traditional standard in rabies diagnosis, the dRIT was 100% sensitive and specific.

The study was carried out in eight dogs slaughtering outlets within four Local Government Areas of the State for the determination of rabies antigen in the saliva and brain of apparently healthy dogs slaughtered for human consumption. A total of one hundred (100) samples each of saliva and brain were collected before and after slaughter, respectively, between April to June, 2013, in the selecte...

1. A direct fluorescent antibody (F.A.) tech­nique for diagnosis of Gonococci is de­scribed. 2. The results of applying F.A. method in the 270 female cases are presented and compared with the conventional method find­ings. 3. 220 cases were diagnosed by one or more methods at the first examinations. F .A. tests alone were positive in 44 ,eases and .F .A. direct diagnosis was made in a tot...

Four stains for the detection of Pneumocystis carinii in respiratory specimens were compared for sensitivity, specificity, preparation time, and ease of interpretation. One hundred specimens were collected. Of these, 50 were induced sputum specimens and 50 were bronchoalveolar lavage fluid. All specimens were stained with Diff-Quik (DQ) (a modified Giemsa stain), a quick silver stain, and direc...

SUMMARY The results of direct stool examination in 102 watery and bloody diarrhea patients were compared with the presence of antibodies against Entamoeba histolytica with Indirect Fluorescent Antibody Test. Besides 22 healthy individuals included in this study as control group. It was found that 22 patients (21.5%) with a sera titre higher than 1/320 bad only 9 (8.8%) positive E. Histolytica ...

introduction babesiosis is caused by apicomplexa parasites of the genus babesia. humans are commonly infected by babesia through tick bites. there is limited information available about babesia infection of humans in china. the aim of this study was to isolate the pathogen from a patient with severe parasitemia. case presentation blood samples were observed by transmission electron microscopy (...

Dynamic and static behavior of the X-H-*-π interactions in X-H-*-π(C6H6) (X = F, Cl, Br, I, HO, MeO, H2N, MeHN and Me2N) is elucidated by QTAIM-DFA (QTAIM dual functional analysis), which we proposed recently, as the first step to clarify various types of X-H-*-π interactions. The asterisk * emphasizes the existence of the bond critical point (BCP) on the interaction in question. Total electron...