In the course of various biological processes, specific DNA-binding proteins must efficiently find a particular target sequence/protein or damaged site on DNA. perform this task based diffusion. Nevertheless, investigations over recent decades have found that diffusion dynamics are generally complicated and, further, protein specific. review, we collect experimental and theoretical studies quan...

In this study, the interaction of the anthracycline antibiotic doxorubicin with DNA-HMG 1 complex was investigated employing UV/VIS spectroscopy, thermal denaturation and DNA cellulose chromatography techniques. The results indicated that the binding of doxorubicin to the protein reduces its Tm in a dose dependent manner. Although doxorubicin protects free DNA against thermal denaturation ...

Nucleic acid-based biosensors for the detection of specific proteins combine typical programmability synthetic DNA systems with artificially controlled DNA-protein communication. The high-affinity interaction between a target protein and ligand, such as an aptamer sequence, or double stranded domain, small peptide, is paired nature-mimicking molecular mechanism allowing probing, processing, tra...

Six genes, including UL32, have been implicated in the cleavage and packaging of herpesvirus DNA into preassembled capsids. We have isolated a UL32 insertion mutant which is capable of near-wild-type levels of viral DNA synthesis; however, the mutant virus is unable to cleave and package viral DNA, consistent with the phenotype of a previously isolated temperature-sensitive herpes simplex virus...

trichophyton rubrum (t. rubrum) is an anthropophilic dermatophyte that is distributed worldwide and causes common cutaneous disease such as mycosis. although several properties of this fungus have been investigated so far, however a few studies were carried out in the field of molecular biology of this fungus. in the present study we tried to identify its molecular characterization of the goano...

background: tumour suppressor genes such as tp53, brca1 and rad51 are involved in dna repair and their malfunctions result in genomic instability and cancer. wild type (wt) tp53 binds to brca1and rad51 in vivo and in vitro. however, mutated tp53 in tumours can interfere with wt tp53 function. we studied how mutation of tp53 in mda-mb-468 cell line could affect its binding capacity and interfere...

in this study, the interaction of the anthracycline antibiotic doxorubicin with dna-hmg 1 complex was investigated employing uv/vis spectroscopy, thermal denaturation and dna cellulose chromatography techniques. the results indicated that the binding of doxorubicin to the protein reduces its tm in a dose dependent manner. although doxorubicin protects free dna against thermal denaturation in th...

LysR-type transcription regulators (LTTRs) comprise one of the largest families transcriptional in bacteria. They are typically homo-tetrameric proteins and interact with promoter DNA ~ 50–60 bp. Earlier biochemical studies have suggested that LTTR binding to bends and, upon inducer binding, bend angle is reduced through a quaternary structure change tetrameric LTTR, leading activation transcri...