Improving labeling probes for state-of-the-art super-resolution microscopy is becoming of major importance. However, there currently a lack tools to quantitatively evaluate probe performance regarding efficiency, precision, and achievable resolution in an unbiased yet modular fashion. Herein, we introduce designer DNA origami structures combined with DNA-PAINT overcome this issue quantification...

Two sets of bioorthogonally applicable, double fluorogenic probes, capable sensing DNA–protein interactions, were prepared by installing an azide or tetrazine motif onto structurally fluorogenic, DNA sensitive frames. Installation these bioorthogonal functions intercalating dyes furnished scaffolds with reactivity based fluorogenicity, rendering probes double-fluorogenic, AND-type logic switche...

To provide rapid and accurate detection of DNA markers in a straightforward, inexpensive, and multiplex format, an alternative surface-enhanced Raman scattering based probe was designed and fabricated to covalently attach both DNA probing sequence and nonfluorescent Raman tags to the surface of gold nanoparticles (DNA-AuP-RTag). The intensity of Raman signal of the probes could be controlled th...

Chromosomal rearrangements are frequently monitored by fluorescence in situ hybridization (FISH) using large, recombinant DNA probes consisting of contiguous genomic intervals that are often distant from disease loci. We developed smaller, targeted, single-copy probes directly from the human genome sequence. These single-copy FISH (scFISH) probes were designed by computational sequence analysis...

In this review, we report on patents concerning self-quenching DNA probes for assaying DNA during or after amplification as well as for direct assaying DNA or RNA, for example in living cells. Usually the probes consist of fluorescently labeled oligonucleotides whose fluorescence is quenched in the absence of the matching target DNA. Thereby the fluorescence quenching is based on fluorescence r...

Combinatorial and modular methods to synthesize small molecule modulators of protein activity have proven be powerful tools in the development new drug-like molecules. Over past decade, these methodologies been adapted toward utilization activity- affinity-based chemical probes, as well chemoproteomic profiling. In this review, we will discuss how like multicomponent reactions, DNA-encoded libr...