Serology and DNA techniques are employed for Human Leukocyte Antigen (HLA) typing in different transplant centers. Results may not always correlate well and may need retyping with different technique. All the patients (with aplastic anemia, thalassemia, and immunodeficiency) and their donors, requiring HLA typing for bone marrow transplant were enrolled in the study. Serological HLA typing was ...

Although variable-number tandem-repeat (VNTR) typing has gained recognition as the new standard for the DNA fingerprinting of Mycobacterium tuberculosis complex (MTBC) isolates, external quality control programs have not yet been developed. Therefore, we organized the first multicenter proficiency study on 24-locus VNTR typing. Sets of 30 DNAs of MTBC strains, including 10 duplicate DNA samples...

This report covers our starting experience on the DNA identification of the post-mortem remains recovered from the battlefields, and illustrates some effects on typing results when STR systems are used for DNA typing of decomposed human remains. This study is primarily concerned with the amplification of degraded DNA samples. Reports of identification by family studies will be presented elsewhere.

We used a sample of Staphylococcus aureus strains that are carried by humans and that are representative of the natural population of S. aureus strains in order to assess the value of multilocus sequence typing (MLST), pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing as epidemiological tools. Only MLST was able to define clonal complexes unambiguou...

Because of the different characteristics of mtDNA compared with nuclear DNA, the application of mtDNA typing and interpretation of results should not in all ways be the same as that routinely practiced for nuclear DNA typing. A higher success rate with typing mtDNA, as opposed to nuclear DNA, from old bones, severely decomposed or charred remains, or single hair shafts is expected and experienc...

Abstract Background The extraction of DNA from skeletal remains with good quality and quantity is often challenging for the ability to generate typing. Previous studies demonstrated total demineralization fresh teeth bones; however, application in old has been less performed. To obtain high yield amount extracted remains, objective this study was focused on exploring factors influencing process...

Saliva is usually deposited in bite marks found in many homicides, assault and other criminal cases. In the present study, saliva obtained from volunteers was deposited on skin and recovered for DNA extraction and typing in order to evaluate its usefulness for practical case investigation and discuss the contribution of forensic dentistry to saliva DNA typing. Twenty saliva samples were collect...

Typing phages for Salmonella and the prophages of their typical propagation strains were analyzed at the DNA level. Most of them belong to the P22 branch of the lambdoid phages. Acquisition of new plating properties of the typing phages by propagation in particular strains can be due to different host specific modifications of the DNA or to recombination events with residing prophages which are...