OBJECTIVE To investigate the feasibility of Descemet stripping automated endothelial keratoplasty (DSAEK) using cultured human corneal endothelial cells (HCECs) in an animal model. METHODS Descemet stripping automated endothelial keratoplasty grafts were produced by seeding cultured HCEC suspensions onto human corneal stromal discs. Three insertion techniques were assessed in an ex vivo model...

PURPOSE To report our clinical experience and 12-month results of small-incision Descemet-stripping endothelial keratoplasty (DSEK). METHODS Prospective study of 11 eyes of 9 patients who had DSEK. The DSEK technique consisted of stripping the Descemet membrane and endothelium from the recipient cornea. The donor button was prepared by manual dissection and inserted through a 5-mm incision. A...

PURPOSE To report the predictability of a nomogram for ultrathin donor lamella creation for Descemet-stripping automated endothelial keratoplasty (DSAEK) with an automated microkeratome. SETTING Instituto de Oftalmología La Arruzafa, Córdoba, Spain. DESIGN Prospective nonrandomized consecutive case series. METHODS This study enrolled eyes of consecutive patients in which DSAEK was perform...

We report a combination of surgical techniques during Descemet-stripping automated endoithelial keratoplasty and intraocular lens (IOL) exchange in patients with pseudophakic bullous keratopathy and angle-supported anterior chamber IOLs. During this procedure, the anterior chamber IOL is exchanged for a posterior chamber iris-claw IOL enclavated to the posterior iris; the anterior chamber is ke...

PURPOSE To determine the immediate endothelial cell loss (ECL) resulting from insertion of a precut donor button using the Neusidl Corneal Inserter (NCI) and compare it with the previously published ECL resulting from insertion of a folded donor button using non-coapting forceps. METHODS Ten corneas were precut for Descemet stripping automated endothelial keratoplasty and trephinated to a dia...

Keratoplasty is considered the most successful organ transplantation procedure in the world. Reisinger was the first to use the term keratoplasty (Reisinger, 1824), when he suggested using an animal’s eye to provide donor corneal tissue for corneal transplantation in a human. In 1906, the first successful penetrating keratoplasty was performed by Edward Konrad Zirm on a patient suffering from b...

357 Corneal transplantation has been widely performed since the first successful surgery was described by Zirm. Recent data from the Eye Bank Association of America (EBAA), the accredited organization that tracks donor quality and volume of surgeries performed anually in the United States, has shown that 46,253 corneal tissues were used for transplantation in 2014 domestically while 76,431 tiss...

PURPOSE The demands for precut lamellar grafts for Descemet stripping automated endothelial keratoplasty rose in our eye bank from 74 in 2007 to 408 in 2010. To meet this expanding requirement, we explored the possibility to preserve these preparations in organ culture. METHODS Organ cultured corneas, stored in a medium containing 6% dextran, were mounted on a Moria artificial anterior chambe...

We describe a case report of using the same handheld spectral-domain anterior segment optical coherence tomography (ASOCT) for rapid intraoperative and postoperative imaging in a case of Descemet membrane endothelial keratoplasty (DMEK). A 67-year-old woman, with Fuchs dystrophy and corneal decompensation, underwent DMEK with intraoperative ASOCT imaging using the handheld Envisu spectral domai...

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