Doubled haploids of carnation (Dianthus caryophyllus L.) were obtained by pseudofertilized ovule culture. Emasculated ̄ower buds of carnation were pollinated with pollen inactivated by Xray irradiation. After 2±3 weeks, the ovaries were explanted and were cultured on solid MS medium containing 2 mM NAA, 2 mM BAP and 6% sucrose. Regenerated plants(R0) were morphologically different from the moth...

Cassava breeding is difficult and, compared with other crops, inefficient. The problems in cassava breeding relate to the length of the breeding cycle, the large genetic load present in the crop, and the heterozygous nature of the parents and progenies evaluated. The production of doubled haploids, through tissue culture techniques, offers interesting advantages. By definition, any process that...

Doubled haploid (DH) technology has been used in breeding programs for several decades and is currently the method of choice in a number of crop species, including barley (Hordeum vulgare L.), rapeseed (Brassica napus L.), maize (Zea mays L.), and wheat (Triticum aestivum L.). In this study we investigated via computer simulation the benefit of using DHs compared with the conventional wheat bre...

By using a doubled-haploid population derived from F2 plants, additive and additive X additive genetic variances, as well as the number of segregating genes, can be estimated. An F2-derived doubled-haploid population may contain almost 50% more of the best recombinant than an F1-derived population. However, the best recombinant occurs in the same frequency in the two populations when there is n...

Quantitative trait loci (QTL) mapping is a powerful tool for investigating the genetic basis of natural variation. QTL can be mapped using a number of different population designs, but recombinant inbred lines (RILs) are among the most effective. Unfortunately, homozygous RIL populations are time consuming to construct, typically requiring at least six generations of selfing starting from a het...