The origin recognition complex (ORC) has an important function in determining the initiation sites of DNA replication. In higher eukaryotes, ORC lacks sequence-specific DNA binding, and the mechanisms of ORC recruitment and origin determination are poorly understood. ORC is recruited with high efficiency to the Epstein-Barr virus origin of plasmid replication (OriP) through a complex mechanism ...

The role of the origin recognition complex (ORC) was investigated in replication initiation and in silencing. Temperature-sensitive mutations in ORC genes caused defects in replication initiation at chromosomal origins of replication, as measured by two-dimensional (2-D) origin-mapping gels, fork migration analysis, and plasmid replication studies. These data were consistent with ORC functionin...

In eukaryotes, DNA replication requires the origin recognition complex (ORC), a six-subunit assembly that promotes replisome formation on chromosomal origins. Despite extant homology between certain subunits, the degree of structural and organizational overlap between budding yeast and metazoan ORC has been unclear. Using 3D electron microscopy, we determined the subunit organization of metazoa...

The origin recognition complex (ORC) specifies replication origin location. The Saccharomyces cerevisiae ORC recognizes the ARS (autonomously replicating sequence) consensus sequence (ACS), but only a subset of potential genomic sites are bound, suggesting other chromosomal features influence ORC binding. Using high-throughput sequencing to map ORC binding and nucleosome positioning, we show th...

In eukaryotes the sites for the initiation of chromosomal DNA replication are believed to be determined in part by the binding of a heteromeric origin recognition complex (ORC) to DNA. We have cloned the genes encoding the subunits of the Drosophila ORC. Each of the genes is unique and can be mapped to discrete chromosomal locations implying that the pattern and developmental regulation of orig...

Crossbred wethers (72; 31 k 0.5 kg) were used to determine effects of different grass sources and qualities on visceral organ mass after 42 and 84days. Wethers consumed ad libitum bermudagrass (BER; Cynodon dactylon) (H, 24-day regrowth; M, 42-day regrowth; L, full-season growth) or orchardgrass (ORC; Dactylis glomeruta) (H, late vegetative to pre-anthesis; M, post-anthesis; L, seed in dough st...

Oral contraceptive (ORC)-related depression and irritability are among the most commonly reported drug-induced psychiatric symptoms. To investigate the etiological role of genetic factors in ORC-related symptoms, we studied questionnaire responses in 715 monozygotic and 416 dizygotic volunteer twin pairs concordant for ORC usage. Biometrical genetic analysis indicated that the liability to ORC-...

BACKGROUND The translation of research into practices has been incomplete. Organizational readiness for change (ORC) is a potential facilitator of effective knowledge translation (KT). However we know little about the best way to assess ORC. Therefore, we sought to systematically review ORC measurement instruments. METHODS We searched for published studies in bibliographic databases (Pubmed, ...

Orc is a new language for task orchestration, a form of concurrent programming with applications in workflow, business process management, and web service orchestration. Orc provides constructs to orchestrate the concurrent invocation of services – while managing timeouts, priorities, and failure of services or communication. In this paper, we show a trace-based semantic model for Orc, which in...

A model to evaluate the performance of a solar powered regenerative Organic Rankine Cycle (R-ORC) using five dry organic fluids: RC318, R227ea, R236ea, R236fa, and R218, is presented in this paper. The system is evaluated in two locations in the U.S.: Jackson, MS and Tucson, AZ. The weather data for each location is used to determine the heat available from the solar collector that could be use...