To the best of our knowledge, there was little information available on pathogen removal using low level disinfectant followed by free chlorine in sequential disinfection (SD). This study investigated Escherichia coli inactivation by four types of disinfection: single step disinfection (SSD), SD, traditional sequential disinfection (TSD) and mixed disinfectant disinfection (MDD). Results indica...

The photocatalytic inactivation of Gram negative Escherichia coli and Gram positive Lactobacillus helveticus by both TiO2 and ZnO with 365-nm ultraviolet (UV) light was studied in a batch reactor. Almost all the initial E. coli cell (10 CFU/ml) were inactivated in 40 min in the presence of 2 g/l ZnO. Photocatalytic inactivation of bacteria was found to follow first order kinetics with the highe...

Rapid and efficient inactivation of a target gene in Escherichia coli chromosomes is required to investigate metabolic engineering. In the present study, a multiple gene inactivation approach was demonstrated in four strains of enterotoxigenic E. coli (ETEC), which are the predominant pathogenic bacteria causing piglet diarrhea, mediated by λ Red and Xer recombination. The chromosomal genes, lu...

Myeloperoxidase (MPO), H2O2, and chloride comprise a potent antimicrobial system believed to contribute to the antimicrobial functions of neutrophils and monocytes. The mechanisms of microbicidal action are complex and not fully defined. This report describes the MPO-mediated inactivation, in Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa, of a class of cytoplasmic membrane...

Pulsed electric field (PEF) technology has been used for the inactivation of microorganisms and to prevent flavor loss in liquid foods and beverages in place of thermal pasteurization. When used to pasteurize orange juice, PEF may prevent loss of volatile sensory attributes. Enterohemorrhagic E. coli O157:H7 (EHEC), two strains of Salmonella Typhimurium, and twenty strains of non-pathogenic bac...

Aconitases are important cellular targets of nitric oxide (NO.) toxicity, and NO.-derived species, rather than NO. per se, have been proposed to mediate their inactivation. NO.-mediated inactivation of the Escherichia coli aconitase and the porcine mitochondrial aconitase was investigated. In E. coli, aconitase activity decreased by approximately 70% during a 2-h exposure to an atmosphere conta...

The ability of power ultrasound, acidic electrolyzed water (AEW), and gamma irradiation to inactivate Escherichia coli O157:H7 inoculated onto alfalfa and broccoli seeds was examined. The treatment conditions under which the alfalfa and broccoli seeds treated with sterile deionized water (SW), AEW, ultrasound cleaning tank (UST), ultrasound probe (USP), and irradiation (IR) would retain a germi...

Zero-valent iron nanoparticles (nano-Fe0) in aqueous solution rapidly inactivated Escherichia coli. A strong bactericidal effect of nano-Fe0 was found under deaerated conditions, with a linear correlation between log inactivation and nano-Fe0 dose (0.82 log inactivation/mg/L nano-Fe0 x h). The inactivation of E. coli under air saturation required much higher nano-Fe0 doses due to the corrosion ...

In this study, effect of different parameters, viz. concentration of photosensitizer (PS), pH of the bacterial cell suspension and initial cell count, on photo-inactivation of Escherichia coli and Enterococcus hirae bacteria using methylene blue (MB) and sodium anthraquinone-2-sulphonate (SAQS) was investigated employing the statistically valid full factorial design of experiments. The inactiva...

The efficient biohybrid photocatalysts were prepared with different weight ratios of Fe2O3 and treated rape pollen (TRP). synthesized samples characterized by analytical techniques. results showed that carbonized had a three-dimensional skeleton granular uniformly covered the surface TRP. Fe2O3/TRP used for degradation Methylene Blue (MB) Escherichia Coli (E. coli) disinfection in water under v...