Eleven children and adolescents with previously normal renal function who received ifosfamide for the treatment of extrarenal solid tumours underwent detailed investigation of glomerular and renal tubular function to assess the incidence and extent of renal damage. None had received cisplatin. Glomerular filtration rate (measured by plasma clearance of 51Cr labelled edetic acid) was reduced in ...

Morphological changes and the content of free carboxyl groups in bovine collagen (type I) film under the influence of trypsin, hydrochloric acid (HCl) and ethylenediaminetetraacetic acid (EDTA) were studied. Incubation with trypsin and HCl was found to cause some delamination of the film and the appearance of some low-density spots. Incubation with EDTA did not cause any morphological changes. ...

We report that the ability of disodium 5'-deoxy-5'-thioguanosine-5'-monophosphate, Na(2)(5'-GSMP), to self-associate into a helical G-quadruplex structure in aqueous solution at pH 8 is significantly higher than that of disodium guanosine-5'-monophosphate, Na(2)(5'-GMP), which supports our earlier hypothesis regarding the importance of cation bridging.

The vital role of ethylenediaminetetraacetic acid on the structure and the oxygen reduction reaction activity of the non-precious-metal-based pyrolyzed catalyst is reported and elaborated. The resultant catalyst can overtake the performance of commercial Pt/C catalyst in an alkaline medium.

The methods used in sample preservation may affect the description of the microbial community structure by DNA-based techniques. This study aims at evaluating the effect of different storage conditions, including freezing, adding two liquid-based preservatives or simply storing samples with no preservative, on the structure of the microbial communities in aliquots of organic-rich soil and water...

Cells grown in suspension culture were incubated with EDTA-disodium salt and shown to have more easily deformable surfaces and raised electrophoretic mobility than controls, following this treatment. The reversibility of these observations by the addition of calcium ions, and other parallel experiments, support the conclusion that, in these cells, calcium is bound to anionic sites at the cell p...

A recombinant Factor VIII (Factor VIII-delta II) consists of a unique polypeptide chain of 165 kDa deleted from the major part of the B-domain and from the cleavage site at Arg-1648-Glu-1649 found in plasma-derived Factor VIII. It was expressed in mammalian cells in serum-free medium containing von Willebrand factor and purified by a one-step immunopurification. The recombinant Factor VIII was ...

Two trials (feedlot and metabolism) were conducted to evaluate the influence of level of chopped tall fescue hay (FH) in high concentrate diets on average daily gain (ADG), liquid and particulate passage rates, digestibility and in situ digestibility of corn. In the feedlot trial, 36 Hereford steers were fed diets containing 15, 30 and 50% FH in combination with 74, 59 and 39% whole shelled cor...