Many recent studies have shown that glycosylation patterns of Agaricus bisporus are similar to those of mammalians, so that this organism is a good candidate for the expression of glycosylated pharmaceutical protein. To achieve constant interested gene expression in all cells of the organism, proper promoter isolation is necessary. To isolate this promoter, PCR with specific primers was perform...

Discarded dried button, dried oyster, and fresh button mushroom beds were examined for their bioactive compounds and industrial enzymes. Levels of reducing sugars and total sugars were high in all mushroom beds. Carboxymethyl cellulase activity was higher (920 μg/mL) in the dried oyster mushroom bed extract. Schizophyllum commune, Fomitopsis feei, Trametes gibbosa, and Trametes elegans were gro...

0

background: white button mushroom (agaricus bisporus) is a sensitive agricultural crop whose antioxidant properties are decreased during postharvest storage. in this study, the effect of citric acid dipping treatment on bioactive components and antioxidant properties of sliced button mushroom (agaricus bisporus) was investigated. methods: fresh mushrooms soaked in chilled citric acid solution a...

dehydration is one of the most common preservation methods for button mushroom. dried mushroom slice and powder is used in formulation of soups and sauces. in this study, the effect of osmotic pretreatment on drying kinetics of button mushrooms was studied. thin slices of mushroom were immersed in osmotic solution containing 5% sodium chloride and 0.5% calcium chloride at a temperature 40 °c fo...

Transferring foreign genes into mushroom mediated by Agrobacterium tumefaciens is a standard technique in genetic engineering. Recombinant human insulin has been greatly used in the treatment of type I diabetes. The production of edible mushroom derived insulin should facilitate oral delivery. In this study we used the Agrobacterium tumefaciens mediated transformation method for the transfer an...

many recent studies have shown that glycosylation patterns of agaricus bisporus are similar to those of mammalians, so that this organism is a good candidate for the expression of glycosylated pharmaceutical protein. to achieve constant interested gene expression in all cells of the organism, proper promoter isolation is necessary. to isolate this promoter, pcr with specific primers was perform...