Nuclei from a monolayer of cultured epithelial cells have been transplanted singly to enucleated unfertilized eggs of Xenopus laevis. About three-quarters of the injected eggs failed to cleave or cleaved abortively. Most of the remainder formed partial blastulae. Less than 1 % reached postneurula stages of development. Nuclei from first-transfer blastulae were used for serial transplantation. T...

This study examined the psychological wellbeing of fathers and father – child relationships in families with a 7-year-old child conceived by donor insemination. Twenty-four donor insemination families and comparison groups of 25 egg donation and 32 unassisted-conception families were assessed using a standardized interview and questionnaires administered to the father, and father – child dyads ...

BACKGROUND Accurate assessments of ovarian reserve (OR) in egg donor candidates are crucial for maximal donor selection. This study assesses whether recently reported new methods of OR assessment by age-specific (as-), rather than non-as (nas-) hormones, follicle stimulating hormone (FSH) and anti-Müllerian hormone (AMH), and triple nucleotide (CGG) repeats on the FMR1 (fragile X) gene have the...

Cryopreservation has the capacity to extend spermatozoa’s lifespan and viability. In addition, the semen samples can be collected, preserved and stored or sent to distant locations and still be used long after the death of the semen donor. In this study for the vitrification of dog sperm (fresh and swum-up sperm), different cryopreservation mediums on the basis of glycerol, milk and egg yolk we...

Background: Despite the meager role of the masculine agent in infertility (the low number of infertile men than women infertile), there are men whose wives are unable to become pregnant due to the absence of sperm, decreased numbers of sperm or lack of sufficient motile sperm. Utilizing donated sperm is a method that enables these families to have children. The use of this method prompts us to ...

In somatic cell nuclear transfer (SCNT), donor cell reprogramming is considered as a biologically important and vulnerable event. Various donor cell pre-treatments with Xenopus egg extracts can promote reprogramming. Here we investigated if the reprogramming effect of one treatment with Xenopus egg extract on donor cells was maintained for several cell passages. The extract treatment resulted i...