One of the tasks of current oncology is identification of cancer stem cells and search of therapeutic means capable of their specific inhibition. The paper presents the data on phenotype characteristics of Ehrlich carcinoma cells as convenient and easy-to-follow model of tumor growth. The evidence of cancer stem cells as a part of Ehrlich carcinoma and significance of CD44+ and CD44- subpopulat...

Tetrocarcin A, isolated from a Micromonospora culture showed activity against experimental i.p. inoculated tumors such as Ehrlich carcinoma, MH134 hepatoma, B16 melanoma. But it was not active against solid tumors such as sarcoma 180 and Ehrlich carcinoma. It was marginally active against the growth of solid Lewis lung carcinoma without prolonging the life span of the tumor-bearing mice. It was...

Several attempts in this laboratory in the last @ or 3 years to culture the Knebs-@ ascites carcinoma of mice had failed. The maximum survival time of the cells in culture was about 10 days. The media used always contained chicken embryo ex tract (CEE). Later, during efforts to develop a culture medium and technic by which single cell cultures could readily be initiated for single-cell irradiat...

Fig. 1. Effect of rifamycins on RNA polymerase reactions of E. coli and Ehrlich carcinoma cells. The reaction mixture for the assay of E. coli RNA polymerase contained (0.3 ml) Tris-HCl, pH 8.0, 15 //moles; /?-mercaptoethanol, 3.6 //moles; MgCl2, 1.2 //moles; MnCl2, 0.3 //moles; ATP, GTP and UTP, 0.1 //mole each; 3H-CTP, 0.05 //moles (3,000 cpm/m/zmole); calf thymus DNA, 30 jug, enzyme, 20jug. ...

The aim of this study is to investigate the antitumor activity and possible molecular mechanism of Phenethyl isothiocyanate (PEITC) against Ehrlich ascites carcinoma in-vivo and in-vitro.In-vivo, ascetic fluid volume, body weight, serum malondialdehyde (MDA) level and total antioxidant capacity (TAC) were determined using Ehrlich ascites carcinoma (EAC) bearing mice. In-vitro, MTT assay was use...

There are 46,000 new cases of peritoneal carcinomatosis per year in the USA and 17,700 in Brazil. New media, including plant derivatives, are being tested in its treatment. Plectranthus amboinicus is a medicinal plant widely used in Brazil, especially in the northeast region, for the treatment of various diseases, including cancer. This present study evaluates the intraperitoneal use of aqueous...

SAITO, T., WAKui, A., KIKKAWA, J., KUDO, T., KIKUCHI, K. and ICHIKAWA, T. Fluctuation of Lipid Content in Ehrlich Carcinoma Cells during the Growth Period. Tohoku J. exp. Med., 1972, 108 (1), 63-77 -1) Total lipid content in Ehrlich ascites carcinoma cells decreased gradually with considerable fluctua tions in the course of cell growth. There was also remarkable fluctuation in individual lipid ...

Several derivatives of mycophenolic acid (MA) were tested for their antitumor activity against leukemia L-1210 when administered orally and carbamoyl mycophenolic acid ethylester (CMAE) was selected as the most active antitumor agent in these tests. An oral administration of CMAE also inhibited the growth of Ehrlich solid carcinoma, NF sarcoma, myeloma X-5563 and sarcoma 180 in mice. In compari...

Many streptomyces strains produced an inhibitor of crude glyoxalase prepared from rat liver which did not inhibit glyoxalase I prepared from yeast. Another inhibitor, C11H14O6, which inhibited glyoxalases prepared from both rat liver and yeast was obtained from a cultured broth of Streptomyces griseosproeus and crystallized. Preincubation of this inhibitor with reuduced glutathione increased it...

Previous studies from this laboratory demonstrated that a potent inhibitor of nucleoside transport, nitrobenzylthioinosine (NBMPR), protected cultured cells against cytotoxic nucleosides (nebularine, tubercidin, and toyocamycin). NBMPR and its 5'-monophosphate (NBMPR-P) also protected mice against potentially lethal dosage of these agents. This report describes protection of mice from potential...