نتایج جستجو برای: electronmicroscopy
تعداد نتایج: 200 فیلتر نتایج به سال:
Subcellular Localization of Glycosphingolipid by Immuno-Electronmicroscopy after Freeze-Substitution
The epithelial and mesothelial cellular components of organs can be obtained as dissociated cells using adequate procedures of enzymatic digestion followed by pycnotic separation on density gradients. Using a specially developed procedure for tissue dissociation, the epithelial and connective tissue components of endometria from pseudopregnant mice were grown in culture using a combination of t...
The isolation of Cowdria ruminantium by means of wheat germ lectin affinity chromatography as described in this paper permits the recovery of partially purified viable organisms under mild conditions in short time. These conclusions are based upon results of analyses of column fractions by intravenous inoculation into sheep, protein determination, electronmicroscopy and enzyme-linked immunosorb...
The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters. New TEM grids coated with ultrathin amorphous Al 2 O 3 films have been developed using atomic layer deposition technique. The amorphous Al 2 O 3 films can withstand temperatures over 600 • C in air and 900 • C in vacuum when the thickness of the Al 2 O 3 film is 2 nm, and up to...
Intact nuclei were isolated from the protoplasts of the filamentous fungus Aspergillus nidulans. The large amounts of protoplasts required for such nuclear preparations were produced from young mycelia grown in liquid culture. For final purification of the crude nuclear fraction, a Nycodenz density-gradient centrifugation was applied. The resulting nuclei were of good purity and morphological s...
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