The electrophoretic behaviour of freshly harvested spores of Aspergillus niger, Phanerochaete chrysosporium and Geotrichum candidum was determined in solution at various pH values. Freshly harvested spores of all three species lacked positive mobility at low pH values, suggesting a preponderance of acidic surface groups. Spores of the ‘non-aggregating’ fungus, G. candidum, had a pH-mobility cur...

The molecules occurring as terminal residues on the external surfaces of nuclei prepared from rat liver by either sucrose-CaCl(2) or citric acid methods and nucleoli derived from the sucrose-CaCl(2) nuclei were studied chemically and electrokinetically. In 0.0145 M NaCl, 4.5% sorbitol, and 0.6 mM NaHCO(3) with pH 7.2 +/- 0.1 at 25 degrees C, the sucrose-CaCl(2) nuclei had an electrophoretic mob...

A gel mobility retardation assay can be used to detect a protein-protein interaction. The assay is based on the electrophoretic mobility of a protein-protein complex being less than that of either protein alone. Electrophoretic mobility is detected by the fluorescence of a green fluorescent protein variant that is fused to one of the protein partners. The assay is demonstrated by using the inte...

The infinite-sites model with no recombination is extended to include mutations that affect electrophoretic mobility. The model allows the effect of a single-site mutation to have a continuous effect on mobility. Formulae are obtained for the variance of electrophoretic mobility of alleles after an arbitrary lenght of time. A special case of the general model is the case of stepwise production ...

The electrophoretic mobilities of low density lipoprotein (LDL) and six pure proteins in a 0.5% agarose gel have been compared to literature electrophoretic mobility values determined by the Tiselius moving boundary method. There is a strong correlation (r = 0.99) between the electrophoretic mobilities determined by the two techniques. The electrophoretic behavior of charged particles smaller t...

Small neutral organic compounds have traditionally the role of markers in electrophoresis, as they are expected to have zero electrophoretic mobility in external electric fields. The background electrolyte contains, however, ions which have unequal affinities to the neutral molecules, which in turn results in their mobilization. In this study we focused on two electrophoretic markers thiourea a...

The three brush-border enzymes--alanine aminopeptidase (EC 3.4.11.2), alkaline phosphatase (EC 3.1.3.1), and gamma-glutamyltransferase (EC 2.3.2.2)--are present in the urine of healthy persons in two variants, a particulate form and a soluble one. They can be separated by electrophoresis in agarose gel and by ultracentrifugation. The particulate forms exhibit similar electrophoretic mobility, b...