BACKGROUND The use of RT-PCR for diagnosis of group A rotaviruses is increasing, but up to 14% of healthy individuals may be positive by RT-PCR. If RT-PCR is not well correlated with disease, rotavirus A may not always be the cause of illness in RT-PCR positive patients with infectious intestinal disease (IID). OBJECTIVES To describe the differences in faecal viral load between ELISA positive...

Aim: To assess the suitability, sensitivity and specificity of F gene based RT-PCR for detection of PPRV infection in India. Materials and Methods: A total of 26 blood samples collected from 26 animals suspected for Peste des petits ruminant (PPR) were tested by RTPCR using F1/F2 primers set and then compared with sandwich-ELISA for detection of PPR virus. Results: Out of 26 samples tested RT-P...

Foot-and-mouth disease (FMD) is one of the highly contagious diseases of domestic animals. Effective control of this disease needs sensitive, specific, and quick diagnostic tools. In this paper seventy suspected field's samples to FMD were analyzed using virus isolation on cell culture, Enzyme Linked Immunosorbant Assay (ELISA), RT-PCR (Reverse TranscriptionPolymerase Chain Reaction) and PCR-EL...

The study was made to find out the etiological agent involved in piglet diarrhoea during 20072009 in and around Bareilly. Group A rotavirus (GARV) was found as the main agent using various methods applied to intestinal and fecal samples. Out of 199 necropsied intestinal samples, none was positive in agarose gel electrophoresis, 5 (2.51%) were positive in double antibody sandwich ELISA (DAS-ELIS...

objective: despite sensitive antibody based blood donor screening, infection can be transmitted during window period. therefore sensetive methods bosed on nucleic acid tests (nats) have been considered. the aim of this project was to design a more sensitive method for detection of pcr products and diagnosis of hiv-1/hcv co-infection accurately. materials and methods: after designing specific p...

Background and Aims: The causal agents of viral lettuce big vein disease are two viruse, Lettuce big vein associated virus (Varicosavirus) and Mirafiori lettuce virus (Ophiovirus). These viruses have coat proteins of similar size but have different morphologies and serologically unrelated.The purpose of this study was to distinguish and detect LBVaV and MiLV in lettuce fields in Tehran Province...

abstract potato virus a (pva) is one of the members of genus potyvirus. pva particles are straight to slightly flexuous rods and contain of single stranded rna. in the spring and summer of 2005, sampling accomplished to determine potato virus a from potato fields in northern and razavi khorasan provinces. samples with mosaic, shining and rolling of leaves carried in ice chamber to laboratory fo...

The bacterium Coxiella burnetii has been associated with reproduction disorders in dairy cattle. A cross-sectional study was conducted Quebec, Canada, to estimate the prevalence of C. cows from RT-PCR-positive and/or ELISA-positive herds. As a secondary objective, associations between burnetii-positivity and three reproductive outcomes (purulent vaginal discharge, cytological endometritis, succ...