Preimplantation genetic diagnosis (PGD) is a novel approach for the prevention of genetic disorders in couples at risk of having offspring with genetic disease. Although the original idea dates back to early 1960s when sexing of rabbit blastocysts was attempted, the first clinical application of PGD was reported about three decades later, describing the use of PCR for sexing embryos from couple...

The objectives of the present study were to establish a nonsurgical transfer method for elongating bovine conceptuses and to combine this method with biopsy and sexing. Bovine conceptuses were recovered from donor cows on days 13-14 of the estrus cycle. In experiment 1, day 13 conceptuses were transferred to recipient cows using a standard day 7 embryo transfer (ET) method. The pregnancy rate o...

My research awarded includes contributions to cryopreservation and sexing of bovine embryos produced in vitro and in vivo, as follows; (1) In vivo-derived morulae and blastocysts were cryopreserved in the presence of 10% glycerol, and the embryos were transferred into recipients after two-step dilution of glycerol in straw, with a practically acceptable pregnancy rate. (2) The survival rate of ...

The ability to obtain genetically identical offspring in cattle (clones) is useful for research and for potential applications to breeding schemes. Experimental possibilities for generating such animals have evolved considerably in the last two decades. Embryo splitting has become a relatively simple technique but is limited to twinning. Embryonic nuclear transfer has improved and is associated...

Artificial insemination (AI) was the first great biotechnology applied to improve reproduction and genetics of farm animals. It has had an enormous impact worldwide in many species, particularly in dairy cattle. The acceptance of AI technology worldwide provided the impetus for developing other technologies, such as cryopreservation and sexing of sperm, estrous cycle regulation, and embryo harv...

Despite the potentials and contributions of guinea fowls to economic and social life in Ghana, accurate sex identification in these birds is still a major problem. Three hundred and sixty guinea fowls (180 birds per sex) were used in determining a more accurate and farmer friendly sexing technique. The sexing methods explored were vent, biometric, and molecular techniques. Vent sexing was accom...