The aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. Blastocyst stage embryos from pregnant NMRI mice were obtained and cultured for 1-2 days in DMEM medium. The inner cell masses formed...

among the female sexual hormones, the estrogen and progesterone are of significant importance. these hormones are prescribed for the treatment of certain female genital system disorders as well as used in the synthesis of contraceptive drugs. it has been shown that administration of the above hor mones during early pregnancy may produce abortion in early gestational days. in the present investi...

embryonic stem cells (escs) are originally derived from the icm of blastocysts and are characterized by their ability to self-renew and their pluripotencies. only a few reports have been published on esc isolations and line establishment in animals, even fewer in horses. however, it is still important to isolate equine escs for animal biotechnology and therapeutic applications. in the present s...

in spite of serious cardiotoxicity side-effects, doxorubicin is frequently used for treatment of several types of cancers. isolated human adult cardiomyocytes could be the best model for assessing drug-induced cardiotoxicity, while harvesting mature cardiomyocytes is restricted by some limitations such as biopsy size, cell numbers, viability, proliferative capacity and their disability to be pa...

embryonic stem cells (escs) are pluripotent, self-renewing cells. these cells can be used in applications such as cell therapy, drug discovery, disease modelling, and the study of cellular differentiation. in this experimental study embryonic stem cells cultured in the laboratory and were amplified. total rna was extracted from cells and converted to cdna by reverse transcription-polymerase cha...

background: high dietary fat consumption may alter oocyte development and embryonic development. this prospective study was conducted to determine the relation between dietary fat consumption level, its food sources and the assisted reproduction parameters. methods: a prospective study was conducted on 240 infertile women. in assisted reproduction treatment cycle, fat consumption and major food...

Early embryonic lung branching morphogenesis is regulated by many growth factor-mediated pathways. Bone morphogenetic protein 4 (BMP4) is one of the morphogens that stimulate epithelial branching in mouse embryonic lung explant culture. To further understand the molecular mechanisms of BMP4-regulated lung development, we studied the biological role of Smad-ubiquitin regulatory factor 1 (Smurf1)...

Public awareness of farm animal welfare has been growing. However, the disclosure, as an important element Corporate Social Responsibility (CSR) reporting, not sufficiently explored. This paper investigates disclosure practices well determinants 2019 corporate reports using a sample Chinese A-share listed food firms. Content analysis is employed for exploring and index adopted instrument conten...

The embryonic myocardium increases functional performance geometrically during cardiac morphogenesis. We investigated developmental changes in the in vivo end-systolic stress-strain relations of embryonic chick myocardium in stage 17, 21, and 24 white Leghorn chick embryos (n = 10 for each stage). End-systolic stress-strain relations were linear in all developmental stages. End-systolic strain ...

Polyembryonic wasps are parasitoids that lay their eggs inside the bodies of other insects. These eggs then undergo a clonal phase of development that results in production of multiple genetically identical offspring. Copidosoma floridanum produces up to 3000 morula stage embryos that form an assemblage called a polymorula. Many aspects of C. floridanum development are well studied but comparat...