The aim of the study was to examine the effect of topical vehicles on the in vivo human stratum corneum penetration of the antioxidant and skin photoprotective agent (-)-epigallocatechin-3-gallate (EGCG). Model oil-in-water (o/w) emulsion and gel formulations containing 1 % (m/m) EGCG were prepared and subjected to photodegradation studies in order to select excipients that minimize the light i...

A new approach to synthesis of cross-linked colloidosomes (microcapsules with a shell from colloidal particles) was developed on the basis of a peroxidized Pickering emulsion (an emulsion stabilized exclusively by peroxidized colloidal particles). Peroxidized latex particles were employed to ensure formation of Pickering emulsion. Free radical polymerization was used to convert droplets of a pe...

A novel technique to estimate the distribution state of carboxyl groups within submicron-sized, carboxylated polymer particles was proposed. For the purpose, carboxyl groups in two kinds of butyl methacrylate-methacrylic acid copolymer emulsion, which were prepared by emulsion copolymerizations with different monomer addition methods, were titrated by isothermal titration calorimetry with an ex...

In situ gelatin zymography is a simple technique providing valuable information about the cellular and tissue localization of gelatinases. Until recently, the use of this technique has been confined to soft, relatively homogeneous tissue. In this report in situ zymography has been utilized to assess the sub-lamellar location of gelatinases in the hard, semi-keratinized epidermal layer and the a...

Reassociating double-stranded DNA from single-stranded components is necessary for many molecular genetics experiments. The choice of a DNA reassociation method is dictated by the complexity of the starting material. Reassociation of simple oligomers needs only slow cooling in an aqueous environment, whereas reannealing the many single-stranded DNAs of complex genomic mixtures requires both a p...

Autoradiographic techniques for use with the electron microscope have been developed and considerably improved in recent years. A review of this historical development is given by Meek and Moses (14), and a theoretical analysis of the problems involved by Pelc (17) and Caro (4). In this paper, we describe a method for preparing autoradiographic specimens, using a new, fine-grained emulsion and ...

Autoradiographic techniques for use with the electron microscope have been developed and considerably improved in recent years. A review of this historical development is given by Meek and Moses (14), and a theoretical analysis of the problems involved by Pelc (17) and Caro (4). In this paper, we describe a method for preparing autoradiographic specimens, using a new, fine-grained emulsion and ...