Background: Autoantibodies against transglutaminase 2 (TG2) are thought to be responsible for the endomysial (EMA), reticulin (ARA), and jejunal antibody (JEA) tissue binding of serum samples from coeliac patients but the exclusive role of TG2 in these staining patterns has not yet been established. Aims: To evaluate whether antigens other than TG2 contribute to EMA/ARA/JEA reactions. Patients:...

BACKGROUND The aim of this study was to retrospectively examine how positive IgA-endomysial antibody (EMA) test results for celiac disease were being interpreted and acted on by physicians in the Calgary Health Region. METHODS We reviewed consecutive EMA test results, with or without a serum IgA, obtained during a 17-month period. Seropositive tests were cross-referenced to the surgical datab...

BACKGROUND Screening for celiac disease is based on the sequential evaluation of serologic tests and intestinal biopsy; an optimal screening protocol is still under investigation. The screening policy of one of the main healthcare providers in Israel (Maccabi) consists of measuring total immunoglobulin A and tissue transglutaminase IgA antibodies and confirming positive results by endomysial an...

the association of celiac disease and type i diabetes mellitus has been known for some time. this study was undertaken to investigate the prevalence of celiac disease (cd) in diabetic children and adolescents. eighty-seven patients (44 females, 43 males) aged 2- 18 years, with type i diabetes participated in this study. a group of 87 healthy unrelated girls and boys matched for age and gender s...

Celiac disease screening test with anti-endomysial antibodies detection is limited by the use of endangered species tissue (monkey distal oesophagus) as well as by the high costs of commercial kits. Due to such limitations new tests are developed to avoid the use of monkey tissue: an IFA test utilising umbilical cord sections (a discarded tissue) and an ELISA test detecting anti-transglutaminas...

background : there is an association   of celiac disease (cd) with several gastrointestinal illnesses. we aimed to determine   the prevalence of cd in patients with inflammatory bowel disease (ibd) to evaluate the value of the routine serological tests for cd in these patients. methods : patients with ibd underwent   screening test for cd. the screening test was based on iga anti-ttg antibody e...

BACKGROUND There is an association of celiac disease (CD) with several gastrointestinal illnesses. We aimed to determine the prevalence of CD in patients with inflammatory bowel disease (IBD) to evaluate the value of the routine serological tests for CD in these patients. MATERIALS AND METHODS patients with IBD underwent screening test for CD. The screening test was based on IgA anti-tTG anti...

AIMS Tissue transglutaminase (tTG) is a major autoantigen recognised by IgA anti-endomysial antibodies (IgA EMA). Enzyme linked immunosorbent assays (ELISA) for IgA anti-tissue transglutaminase antibodies (IgA tTG) have therefore been developed as an alternative serological screening test to IgA EMA for coeliac disease (CD). The use of human tTG (h-tTG), as opposed to guinea pig liver tTG (gpl-...

BACKGROUND Coeliac disease (CD), due to its protean clinical manifestation, is still very under diagnosed in adults and delays in diagnosis may take years and even decades. Simple tools to find cases in primary care may help to identify patients for further diagnostic tests. We have evaluated the usefulness of an on site rapid fingertip whole blood point-of-care test (POCT) for such a purpose. ...

Aim: To determine the prevalence of celiac disease related autoantibodies in patients with type 1 and 2 diabetes mellitus (DM), and to compare these results with the general population. Methods: In total, 137 consecutive patients with type 1 DM, 172 with type 2 DM and 113 age-sex matched control subjects were included into the study. Antigliadin-autoantibodies (AGA) IgG and IgA, and endomysial-...