Introduction. Infections associated with carbapenem-resistant (Carba-R) Klebsiella pneumoniae (KP) are a serious public health problem because they lead to an increase in hospital stays, treatment costs, and patient morbidity mortality.
 Aim characterize antimicrobial resistance, virulence factors genotypes of Carba-R KP strains isolated from patients intensive care surgical units.
 M...

Strains of Salmonella enterica lacking YggX and the cellular reductant glutathione exhibit defects similar to those resulting from iron deficiency and oxidative stress. Mutant strains are sensitive to hydrogen peroxide and superoxide, deregulate the expression of the Fur-regulated gene entB, and fail to grow on succinate medium. Suppression of some yggX gshA mutant phenotypes by the cell-permea...

ATP up-regulates macrophage antimycobacterial activity in a P2X7-dependent manner, but little is known about whether ATP directly exhibits antimicrobial effects against intracellular mycobacteria. In this study, we found that ATP inhibited the growth of various bacteria, including Staphylococcus, Pseudomonas, and mycobacteria, without damaging bacterial surface structures. Using gene technology...

The goal of this research was to evaluate the functional properties and safety antilisterial Enterococcus faecium BT29.11 isolated from Turkish Beyaz cheese. E. showed highest inhibitory activity against Listeria monocytogenes, followed by Staphylococcus aureus vancomycin-resistant enterococci. identified 16S rDNA sequence analysis, genus- species-specific PCR. entA, entB, entX structural genes...

This study aimed to isolate and identify different Enterococcus spp. from diverse sources including dromedary raw milk, rhizospheric soil natural saline water samples determine their antimicrobial activities safety aspects. From these sources, distinct strains of E. faecium, durans, hirae, gallinarum species were detected. No virulence factors observed for six while none the tested Enterococci ...

Cerulenin is an antibiotic that inhibits fatty acid synthesis by covalent modification of the active thiol of the chain-elongation subtypes of beta-ketoacyl-acyl carrier protein synthase. It also inhibits other processes that utilize essential thiols. Cerulenin has been widely reported to block protein secretion at sub-MIC levels, an effect that has been postulated to represent interference wit...

Klebsiella pneumonia infection rates have increased dramatically. Molecular typing and virulence analysis are powerful tools that can shed light on Klebsiella pneumonia infections. Whereas 77.7% (28/36) of clinical isolates indicated multidrug resistant (MDR) patterns, 50% (18/36) indicated carpabenem resistance. Gene prevalence for the AcrAB efflux pump (82.14%) was more than that of the mdtK ...

In order to identify genes belonging to the Fur regulon of Salmonella typhimurium, a bank of 10,000 independent S. typhimurium MudJ insertion mutants was screened for lacZ fusions regulated by the iron response regulator Fur. In parallel, a plasmid gene bank of S. typhimurium consisting of 10,000 independent clones was screened for Fur-regulated promoters or iron binding proteins by the Fur tit...

Screening of lactic acid bacteria (LAB) from Tunisian raw bovine milk has resulted in the isolation of an Enterococcus italicus strain designated as GGN10, which has demonstrated a bacteriocin-like activity against Listeria spp. Antibacterial activity in the culture supernatant was lost after treatment with proteolytic enzymes, whereas it was stable over a wide range of pH (2–10). The treatment...