Adequate comparisons of DNA and cDNA libraries from complex environments require methods for co-extraction of DNA and RNA due to the inherent heterogeneity of such samples, or risk bias caused by variations in lysis and extraction efficiencies. Still, there are few methods and kits allowing simultaneous extraction of DNA and RNA from the same sample, and the existing ones generally require opti...

Vanillin was extracted from vanilla beans using pretreatment with cellulase to produce enzymatic hydrolysis, and response surface methodology (RSM) was applied to optimize the processing parameters of this extraction. The effects of heating time, enzyme quantity and temperature on enzymatic extraction of vanillin were evaluated. Extraction yield (mg/g) was used as the response value. The result...

14 Regulatory, safety, and environmental issues have prompted the development of aqueous 15 enzymatic extraction (AEE) for extracting components from oil-bearing materials. The 16 emulsion resulting from AEE requires de-emulsification to separate the oil; when enzymes 17 are used for this purpose, the method is known as aqueous enzymatic emulsion de18 emulsification (AEED). In general, enzyme a...

The demand of fruit juices is increasing day by day as a result of increasing health awareness among the people. Most of juice extraction processes are not producing satisfactory quantity and quality of juices. Enzymatic extraction of juices results in higher yield. The enzymes used are mainly pectinase, cellulase, hemicellase etc. Apart from promoting juice extraction, enzyme addition increase...

Antibody production and affinity maturation are driven by B cell extraction and internalization of antigen from immune synapses. However, the extraction mechanism remains poorly understood. Here we develop DNA-based nanosensors to interrogate two previously proposed mechanisms, enzymatic liberation and mechanical force. Using antigens presented by either artificial substrates or live cells, we ...

Many of the alkaloids used as drugs today are from natural sources. These drugs have been modified to produce analogs for clinical use. One of these alkaloids, pilocarpine, is an imidazole alkaloid found in plants of the genus Pilocarpus (Fig. 1). Plants of this genus are designated by the name jaborandi but only Pilocarpus microphyllus, which accumulates the highest pilocarpine content, is con...

Please cite this article in press as: Latif, S., & j.foodchem.2010.09.064 In the present work we evaluated five enzyme-mixtures (Protex 7L, Alcalase 2.4L, Viscozyme L, Natuzyme, and Kemzyme) for their effectiveness in extracting the oil and protein recovery from sesame seeds during an enzyme-assisted aqueous extraction (EAAE) process. Alcalase 2.4L was found to be the best for attaining a high ...

Fruits and vegetables are considered a good source of polyphenols and antioxidant capacities which are beneficial in protecting the human body against damage induced by reactive species. The objective of this work is to conduct an assessment of the polyphenol content and antioxidant activities of different fruit (kiwi, pear, green apple, raspberry, blackberry, strawberry and blueberry) and vege...

Accepted: 21/03/2014 Abstract Enzymes are an integral component of modern fruit juice manufacturing processes. Their main functions are to: increase extraction of juice from raw material, increase processing efficiency (pressing, solid settling or removal), and generate a final product that is clear and visually attractive. Juice extraction can be done by using various mechanical processes, whi...