background: in patients with non-obstructive azoospermia (noa), vital spermatozoa from the tissue is obtained from testes by enzymatic treatment besides the mechanical treatment. objective: to increase the sperm recovery success of testicular sperm extraction (tese), with enzymatic digestion if no sperm is obtained from testis tissue by mechanical method. materials and methods: tissue samples w...

organophosphorus compounds are highly toxic chemicals that have application in production of warfare agents and agricultural pesticides. detoxification of areas that have been exposed to these types of materials is of vital importance and several approaches have been used for this purpose. biodegradation of organophosphates is one of these approaches that is in the center of attention due to it...

The Enzymatic Mutation Detection (EMD) assay detects mutations or polymorphisms in DNA. The assay procedure takes <1 h and is followed by electrophoretic detection. We report an automated procedure, using fluorescently labeled probe and quantitative analysis on the ABI Prism 377 DNA Sequencer, that improves on earlier methods (1, 2) by eliminating the need for sample purification, shortening th...

Given the widespread interest in antioxidant nutrients, we have developed a new procedure that will permit the automated determination of plasma ascorbic acid (AA) concentration with a Roche Fara centrifugal analyzer. After the deproteinization of plasma with metaphosphoric acid, AA is oxidized to dehydroascorbic acid by AA oxidase. The product is coupled to o-phenylenediamine to produce a chro...

The glucoseoxidase-peroxidasesystem for the enzymatic determination of glucose was investigated with respect to the instability of the enzyme reagent, the buffering capacity of the reaction milieu, and the stability and sensitivity of the final chromogen. An improved procedure was developed in which the enzyme solution is both stabilized and more adequately buffered by reconstitution in 40% aqu...

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* Supported in part by a grant from the Atomic Energy Commission, Contract No. AT (30-1) 1017. t Public Health Research Fellow of the National Cancer Institute. 1 Yanofsky, C., J. Biol. Chem., 223, 171 (1956). 2 Yanofsky, C., and M. Rachmeler, Biochim. et Biophys. Acta, 28, 640 (1958). 3 DeMoss, J. A., M. Imai, and D. M. Bonner, Bact. Proc., 112 (1958). 4Yanofsky, C., Biochem. et Biophys. Acta,...

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We evaluated a new analyzer that measures lactate in undiluted whole blood by direct (or undiluted) amperometry [Nova Stat Profile 7 Analyzer (SP7); Nova Biomedical, Waltham, MA] by comparing it with two other analyzers, one for measuring lactate in whole blood by indirect (or diluted) amperometry [Model 2300; Yellow Springs Instrument Co. (YSI), Yellow Springs, OH] and another for measuring la...

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