Immobilization of Lipase produced from Rhizomucor miehei on HDPE fine powder was investigated. As compared to an aqueous system, immobilization in a non-aquous organic medium such as n-hexane was not successful and caused enzyme denaturation. Prewetting the support with ethanol increased the immobilized protein and enzyme activity as much as 31% and 34%, respectively. The maximum immobilized a...

the binding behavior of a competitive inhibitor, n-phenylbenzhydroxamic acid (bha) against horseradish peroxidase (hrp) was studied in order to understand and predict the interaction mechanism of hydrogen donors with the enzyme. the dissociation constants of the complexes of hrp-bha, hrp-donor and hrp-bha-azide were estimated at specified conditions by difference spectroscopy. the binding site ...

Copper amine oxidases (CAOs) catalyse the oxidative de-amination of biogenic amines which are ubiquitous compounds essential for cell growth and proliferation. The enzymes are homodimers containing both topaquinone and a Cu(II) ions as cofactors at the active site of each subunit. After extraction and purification of chickpea (cicer arietinum) amine oxidase by chromatoghraphy, Km and Vmax of th...

Deubiquitinating enzymes (Dubs) function to remove covalently attached ubiquitin from proteins, thereby controlling substrate activity and/or abundance. For most Dubs, their functions, targets, and regulation are poorly understood. To systematically investigate Dub function, we initiated a global proteomic analysis of Dubs and their associated protein complexes. This was accomplished through th...

T he metabolic studies described in the previous article represent a relatively straightforward application of NMR spectroscopy. The positions of the various resonances and their heights allow one to determine the presence and amount of metabolic intermediates and products and, in turn, the specific metabolic pathways involved. These tracer studies with stableisotope labels are thus directly an...

Force field and first principles molecular dynamics simulations on complexes of pig liver esterase (pig liver isoenzymes and a mutant) and selected substrates (1-phenyl-1-ethyl acetate, 1phenyl-2-butylacetate, proline-β-naphthylamide and methyl butyrate) are presented. By restrained force field simulations the access of the substrate to the hidden active site was probed. For a few substrates sp...

A 2 × 3 factorial experiment was conducted to investigate the effect of enzyme Endofeed W (EEW) at 0 and 0.05%, thyme essential oil (TEO), probiotic Primalac® (PP) and non-feed additive, on intestinal histomorphology of broilers fed wheat-based diet. Each of the 6 dietary treatments was replicated 4 times with 12 chicks each from 0 to 42 days of age. There was no interaction between enzyme and ...