?2 microglobulin (?2m) fibril has been recognized as the major factor of dialysis-related amyloidosis. elevated serum levels are thought to be the basis for tissue deposition. in order to evaluate the effect of cellulosis membrane (fb 110) and treatment duration on s?2m levels, we determined serum levels in 23 hemodialyzed patients with end-stage renal failure. s?2m levels were markedly elevate...

The intracellular localization of the post-translationally inserted integral membrane protein, NADH-cytochrome b5 reductase, was investigated, using a quantitative radioimmunoblotting method to determine its concentration in rat liver subcellular fractions. Subcellular fractions enriched in rough or smooth microsomes, Golgi, lysosomes, plasma membrane and mitochondrial inner or outer membranes ...

Polyacrylonitrile (PAN) membranes have attracted much attention due to a variety of excellent characteristics, which include thermal stability nd tolerance to most solvents, atmosphere, bacteria and photo irradiation. Therefore, their exploitation also covers many fields, like pervaporation, ater treatment, enzyme immobilization, and hemodialysis. However, the relative poor hydrophilicity and b...

premature rupture of membranes (prom) is one of the most common complications of pregnancy that has a major impact on neonatal outcomes. with respect to racial, nutritional and cultural differences between developed and developing countries, this study was conducted to detect the prevalence of neonatal complications following prom and the role of the duration of rupture of membranes in producin...

Lectin inactivation of the cell surface enzyme 5'-nucleotidase has been studied in intact 13762 mammary ascites cells, cell membrane envelopes, membrane vesicles, and solubilized membranes as a means of understanding the effects of lectins on biochemical processes and the behavior of the enzyme in the membranes. The properties of the enzyme are essentially the same in the intact cells and in me...

Soluble and membrane associated proline dehydrogenase differ in catalytic properties. The soluble enzyme transfers electrons from L-proline to exogenous electron acceptors. It has a high Km for L-proline (105 mM) and is insensitive to the respiratory chain inhibitors 5-ethyl-5-isopentyl-barbituric acid and cyanide. The membrane-associated enzyme transfers electrons from L-proline to O2 via the ...

1. The enzyme which decomposes the capsular polysaccharide of Type III Pneumococcus is associated with a protein which under optimal conditions of filtration passes through membranes with an average pore size of 10.6 mmicro but is held back by pores having a diameter of 8.2 mmicro. 2. When enzyme solutions are filtered to dryness through membranes of such porosity as to hold back the active pri...

Kinetic properties of Na+, K+ ATPase of membranes from rat and human erythrocytes were examined. The enzyme stability decreased with incubation time. The Vmax of the human enzyme was about 4 times lower than the values of the rat enzyme. However the energies of activation were higher. Phase transition temperature for the rat and the human enzyme was 24 degrees C and 17 degrees C, respectively. ...

the gas separation performance of commercial ceramic titania and hybrid silica (hybsi®) membranes was assessed using the gas permeation and permporometry methods. results indicated that the hybsi® membranes have a hybrid surface containing regions covered by a polymeric matrix and others with inorganic pores. these membranes have high h2 selectivity, which increases significantly with temperatu...

The intrinsically active, sn-glycerol-3-phosphate acyltransferase present in membranes prepared from both wild type Escherichia coli and from strains which overproduce the enzyme can be kinetically distinguished from a latent enzyme species which is unmasked by solubilization and reconstitution. Both membrane-associated and solubilized/reconstituted enzyme preparations exhibited cooperativity w...