S-2,3-Dicarboxyaziridine was found to be a potent competitive inhibitor (Ki = 0.08 microM) of fumarase from pig heart. The aziridine did not inactivate the enzyme or exhibit any observable substrate activity. It is likely that it functions as a transition state analogue mimicking the carbanion intermediate found in the normal catalytic reaction. The aziridine inhibited fumarate utilization in r...

Angioedema of the small bowel (ASB) is an extremely rare side effect of the angiotensin-converting enzyme inhibitors (ACEI). We present a case of ACEI-induced ASB mimicking postoperative complication. The diagnosis of ACEI-induced ASB should be considered in patients using these drugs and presenting sudden gastrointestinal symptoms and thickening of small bowel not attributable to other diseases.

Mimicking natural photosynthesis by direct photoelectrochemical (PEC) reduction of CO2 to chemicals and fuels requires complex cell assemblies with limitations in selectivity, efficiency, cost, stability. Here, we present a breakthrough cathode utilizing an oxygen tolerant formate dehydrogenase enzyme derived from clostridium carboxidivorans coupled novel efficient situ nicotinamide adenine din...

Protocells containing enzyme-driven biomolecular circuits that can process and exchange information offer a promising approach for mimicking cellular features developing molecular platforms. Here, we employ synthetic transcriptional together with CRISPR/Cas-based DNA processing inside semipermeable protein-polymer microcompartments. We first establish protocell be activated by external strands ...

Significance Photosynthesis uses chlorophylls to utilize solar energy. In oxygenic phototrophs, formation of the isocyclic fifth ring chlorophyll, responsible for its green color, is catalyzed by AcsF/CycI and auxiliary protein Ycf54. Removal Ycf54 causes severe chlorophyll deficiency impaired photoautotrophic growth. We analyzed laboratory-evolved suppressor mutants a Ycf54-less strain cyanoba...

Artificial enzymes with modulated enzyme-mimicking activities of natural systems represent a challenge in catalytic applications. Here, we show the creation artificial Cu metalloenzymes based on generation nanoparticles an enzyme matrix. Different were used, and structural differences between especially influenced controlled size environment that surrounds them. Herein, demonstrated oxidase-lik...

BACKGROUND The aim of this study was to investigate the frequency of autoantibodies with mimicking specificity by using the dilution technique, to assess the usefulness of the combination of the dilution technique and red blood cell (RBC) phenotyping, and to establish a pre-transfusion testing algorithm in patients with warm autoantibodies. METHODS Serum samples from 71 patients with warm aut...