Background: Foot-and-mouth disease (FMD) is a highly contagious and economically important disease that affects cloven-hoofed animals worldwide. In recent years, a series of outbreaks of FMD have occurred in many countries. Recombinant protein synthesis incorporating protective B- and T-cell epitopes are candidates for new safer and more effective (FMD) vaccines that have potential to provide p...

Aspergillus fumigatus is a pathogenic microorganism that causes aspergillosis due to the presence of its allergenic proteins. During last two years, few clinical cases have been reported where allergic bronchopulmonary (ABPA) has detected in COVID-19 patients. The administration antifungal medicine did not provide satisfactory results. It challenging job for medical scientists protect mankind b...

Rational design of peptide vaccines becomes important for the treatment of some diseases such as Alzheimer's disease (AD) and related disorders. In this study, as part of a larger effort to explore correlations of structure and activity, we attempt to characterize the doubly phosphorylated chimeric peptide vaccine targeting a hyperphosphorylated epitope of the Tau protein. The 28-mer linear chi...

Antibodies play an important role in the natural immune response to invading pathogens. The strong and specific binding to their antigens also make them indispensable tools for research, diagnostics and therapy. This thesis describes the development of methods for characterization of antibody specificity and the use of these methods to investigate the polyclonal antibody response after immuniza...

Abstract Background Hemagglutinin is a major surface protein in influenza A virus (IAV), and HA2 relative conserved among different IAVs. It will be meaningful to identify broad-spectrum epitopes based on the protein. Results Overlapping peptides of H5N1 IAV A/Mallard/Huadong/S/2005 were synthesized loaded modified silica gel film form microarray, antisera against subtypes IAVs used screen univ...

INTRODUCTION Epstein-Barr virus exposure appears to be an environmental trigger for rheumatoid arthritis that interacts with other risk factors. Relationships among anti-cyclic citrullinated peptide antibodies, the shared epitope, and smoking status have been observed in patients with rheumatoid arthritis from different populations. OBJECTIVE To perform an association analysis of anti-Epstein...

T cell immune responses are driven by the recognition of peptide antigens (T cell epitopes) that are bound to major histocompatibility complex (MHC) molecules. T cell epitope immunogenicity is thus contingent on several events, including appropriate and effective processing of the peptide from its protein source, stable peptide binding to the MHC molecule, and recognition of the MHC-bound pepti...

The venom toxin δ-ctenitoxin-Pn2a of the spider Phoneutria nigriventer can cause severe envenomation in humans. Furthermore, cystine-knot motif provides exceptional stability, thereby hampering immune response activation. Here we identified epitope G34YFWIAWYKLANCKK48 from through Immune Epitope Database Analysis Resource and used it to design antigenic peptides. Cys residue was replaced by α-a...

The immunogenicity of a chimeric T/B cell peptide corresponding to antigenically characterized epitopes of the Chlamydia trachomatis major outer membrane protein (MOMP) was studied in mice to further define its potential use in the development of a subunit vaccine in preventing blinding trachoma in humans. The chimeric peptide, designated A8-VDI, corresponds to a conserved MOMP T helper (Th) ce...

Peptide vaccines containing minimal epitopes of protective Ags provide the advantages of low cost, safety, and stability while focusing host responses on relevant targets of protective immunity. However, the limited complexity of malaria peptide vaccines raises questions regarding their equivalence to immune responses elicited by the irradiated sporozoite vaccine, the "gold standard" for protec...