ABSTRACT Current knowledge about microbial injury was derived mostly from studies with bacteria associated with processed foods. Demonstration of injury and repair in phytopathogenic bacteria and its implication on pathogen detection and disease ecology have not been reported. The objective of this study was to investigate the lethal and sublethal effects of acetic acid (AA) on soft-rot Erwinia...

The lipopolysaccharide of Erwinia carotovora was analyzed by quantitative sugar analysis, methylation analysis, and chromic oxide oxidation. This led to the following structure of the core oligosaccharide: (Formula; see text).

The present study investigates antibacterial activity of tobacco extracts from Nicotiana tabacum at different concentrations in different polar solvents. Six different extracts were prepared, using 5 different polar solvents viz., Ethanol, Ethyl acetate, n-Hexane, Acetone, Butanol and water. Four different concentrations (6, 12, 18 and 24 mg of sample disc) of each extract were subjected for pr...

The polygalacturonase, clearly differentiated from polygalacturonic acid trans-eliminase, produced in the culture fluid of Erwinia carotovora was purified 56-fold by means of acetone precipitation, acetate butfer extraction, and two successive treatments by chromatography on carboxymethyl cellulose. The purified enzyme is specific for nonmethoxylated polygalacturonic acid. It shows an optimum p...

Using mini-Tn5CmR::gusA, a transposon that allows transcriptional fusions to a promoterless beta-glucuronidase gene, a mutant of Erwinia carotovora subsp. carotovora SCC3193 deficient in extracellular protease production and soft-rot pathogenicity in plants was isolated. The mutant, designated SCC6004, produced normal levels of pectate lyase, polygalacturonase and cellulase. The region of the t...

The production of antibiotics and their role in microbial competition under natural conditions can be readily studied by the use of transposon mutants. Several antibiotic-producing strains of Erwinia carotovora subsp. betavasculorum were unable to accept foreign DNA. A plasmid delivery system was developed, using ethyl methanesulfonate mutagenesis, which entailed isolating E. carotovora subsp. ...

Erwinia carotovora are phytopathogenic Gram-negative bacteria of agronomic interest as these bacteria are responsible for fruit soft rot and use insects as dissemination vectors. The Erwinia carotovora carotovora strain 15 (Ecc15) is capable of persisting in the Drosophila gut by the sole action of one protein, Erwinia virulence factor (Evf). However, the precise function of Evf is elusive, and...