OBJECTIVE The objective of the study was to evaluate the effectiveness of chitosan and chitosan-ethylenediamine tetraacetic acid (EDTA) (3:1,1:1,1:3) in comparison with 5.2% sodium hypochlorite (NaOCl) in disinfecting Enterococcus faecalis biofilm on root canal dentin and in the removal of smear layer with minimal erosion. MATERIALS AND METHODS Seventy single-rooted extracted human mandibular...

Interest in ethylenediaminetetraacetic acid (EDTA) arises from its ability to combine with metal ions to form water-soluble chelates. Several important biological applications have been made of this property. The material has been shown to be effective (a) in mobilizing radioactive elements such as Pu, Am, and Y from the skeleton (1, 2), (b) as a valuable therapeutic agent in the treatment of p...

Hirudin, an extract from the leech, has powerful antithrombin activity affecting the blood coagulation pathway. We evaluated the usefulness of hirudin in anticoagulating specimens for routine laboratory tests. Results using blood anticoagulated with hirudin corresponded well with results with blood treated with ethylenediamine tetraacetic acid (EDTA) in the complete blood count (CBC), including...

By means of a continuous canine kidney cell line (MDCK), influenza viruses were rapidly isolated from specimens collected from patients with respiratory disease. The cell line proved more sensitive than either eggs or rhesus monkey cells for currently circulating influenza A and B strains. Influenza viruses caused a distinct cytopathology within 5 days of inoculation if trypsin-ethylenediamine-...

In experiments on digestive function in ruminants, markers can be used to estimate the rate of movement of digesta, the volume of a viscus and the rates of absorption of solutes from the gut. The most widely used water-soluble marker is polyethylene glycol (PEG), which was introduced by Sperber, HydCn & Ekman (1953). They found that the polymer with mean molecular weight 4000 was not degraded i...

Ethylenediaminetetraacetic acid, ethylenediamine-N,N'-disuccinic acid and ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid are polyaminocarboxylic acids that are able to sequester metal ions. Calcium is implicated in maintenance of intercellular matrix, zonula occludens (tight junctions) and zonula adherens of epithelium and endothelium cells. Corneal epithelium is impervious t...

Four tests for the evaluation of lead poisoning are reviewed from both the clinical and methodological aspects. Whole blood or erythrocyte lead measurements appear to provide the best means of assessing the bodily burden of lead with electrothermal and Delves cup flame atomic absorption spectorphotometric techniques providing accurate and precise results. Urine lead is less reliable as a screen...

OBJECTIVES The purpose of this study was to evaluate the effect of chelation therapy with ethylenediamine tetraacetic acid (EDTA) on endothelium-dependent vasomotor responses in patients with documented coronary artery disease (CAD). BACKGROUND Oxidative stress plays an important role in the dysfunction of endothelium and development of atherosclerosis. Modification of cardiac risk factors an...

Chemicals and materials NaClO4, Pb(ClO4)2·3H2O, KClO4·3H2O, K3[Fe(CN)6], K4[Fe(CN)6], 6-mercaptohexanol, Tris(tris-(hydroxymethyl)-aminomethane), dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich Inc. Ethylenediamine tetraacetic acid (EDTA), Ethylenediamine tetraacetic acid dipotassium salt (EDTA-2K) were purchased from J&K Scientific Ltd. Mg(ClO4)2 was purchased from Fluka. The oligo...