Decolorization of brilliant green (0.06 g/L), Evans blue (0.15 g/L), and their mixture (total concentration 0.08 g/L, proportion 1:1 w/w) by fungi was studied. Fungal strains [Pleurotus ostreatus (BWPH), Gloeophyllum odoratum (DCa), and Fusarium oxysporum (G1)] were used separately and as a mixture of them. Zootoxicity (Daphnia magna) and phytotoxicity (Lemna minor) changes were estimated after...

The influence of chronic arterial hypertension upon the permeability to albumin of the cerebral capillaries, i.e. the blood-brain barrier, was studied in normotensive Wistar and spontaneously hypertensive Wistar rats with experimental subarachnoid hemorrhage. The blood-brain barrier permeability to albumin was assessed quantitatively by spectrophotometric determination of Evans blue extravasati...

A cleavable linker strategy has been used to optimise the enolate alkylation reactions of a recyclable L-tyrosine derived polymer-supported oxazolidin-2-one for the asymmetric synthesis of a series of chiral alpha-alkyl acids.

BACKGROUND AND PURPOSE We sought to validate the blood-brain barrier permeability measurements extracted from perfusion-weighted MRI through a relatively simple and frequently applied model, the Patlak model, by comparison with gold standard histology in a rat model of ischemic stroke. METHODS Eleven spontaneously hypertensive rats and 11 Wistar rats with unilateral 2-hour filament occlusion ...

The methods at present available for the determination of the dye, Evans blue, in plasma have certain disadvantages. Direct colorimetry of dyed plasma is inapplicable where the plasma is haemolyzed, turbid or lipaemic. The butanol extraction procedure of Harington, Pochin & Squire (1940) is troublesome and time-consuming where many analyses must be carried out; its accuracy is lower than those ...

OBJECTIVES This study evaluated subcutaneous tissue response to Aroeira (Myracrodruon urundeuva) extract employing edemogenic and histological analyses. MATERIAL AND METHODS Test groups consisted of aqueous and ethanolic Aroeira extracts and saline (control). For edema quantification, 18 rats received an intravenous injection of Evan's Blue. After 30 min, the extracts and saline were injected...

Sorsby (I939) used intravenous Kiton green to produce permanent vital staining of the diseased human retina. Other workers have used vital staining dyes in experimental animals. Steinhausen and Loreth (I965) used Lissamin green in investigating the rat retina. Riehm and Podesta (I97 I) used both Patent blue (Disulphine blue) and Lissamin green in an examination of choroidal blood flow in an exp...

OBJECTIVE Loss of endothelial barrier function in arterial blood vessels is characteristic of vascular pathologies, including atherosclerosis. Here, we present a near-infrared fluorescence (NIRF) imaging methodology for quantifying endothelial permeability and macromolecular uptake in large arteries in the mouse and evaluate its applicability for studying mechanisms of vascular inflammation. ...

Quantitation of cardiac output by the Stewart-Hamilton method will have extended usefulness if the accuracy of measuring the concentration of Evans blue dye in whole blood is established. Cardiac outputs by this method have been compared in dogs with outputs by the rotameter and the Tick method. The constant-flow photometer (Friedlich) was used initially, but certain difficulties in its use led...