Even with decreasing DNA synthesis costs there remains a need for inexpensive, rapid, and reliable methods for assembling synthetic DNA into larger constructs or combinatorial libraries. Advances in cloning techniques have resulted in powerful in vitro and in vivo assembly of DNA. However, monetary and time costs have limited these approaches. Here, we report an ex vivo DNA assembly method that...

427 LE P OI NT D E VU E DE S IR IU S FO RU M Ayant eu le privilège d’assister au Symposium International sur les cellules ES humaines (FISH-ESC) organisé par Marc Peschanski (Genocentre d’Évry, 31 janvier-2 février 2008) (➜), je voudrais le replacer dans le contexte de la révolution de la biologie moléculaire et de son impact médical. La plupart des gènes de maladies humaines et leurs mutations...

Ex-vivo brain quantitative susceptibility mapping (QSM) allows investigation of brain characteristics at essentially the same point in time as histopathologic examination, and therefore has the potential to become an important tool for determining the role of QSM as a diagnostic and monitoring tool of age-related neuropathologies. In order to be able to translate the ex-vivo QSM findings to in-...

BACKGROUND Advanced organ failure is often classified as an end-stage disease where the treatment options are limited only to transplantation. As an alternative, different attempts have been undertaken to improve the outcome of the treatment of failing organs by using targeted ex vivo approaches. This may solve the issue of organ shortage by treating the donor organs before transplantation and ...