Lung transplantation offers a lifesaving therapy for patients with end-stage lung disease but its availability is presently limited by low organ utilization rates donor lungs frequently excluded due to unsuitability at assessment. When does occur, recipients are then vulnerable primary graft dysfunction (PGD), multitudinous short-term complications, and chronic allograft dysfunction. The decisi...

OBJECTIVE To evaluate the use of ex vivo lung perfusion (EVLP) clinically to prepare donor lungs for transplantation. METHODS A prospective study involving EVLP for the reconditioning of extended-criteria donor lungs, the criteria for which include aspects such as a PaO2/FiO2 ratio < 300 mmHg. Between February of 2013 and February of 2014, the lungs of five donors were submitted to EVLP for u...

Damaged lung grafts obtained after circulatory death (DCD lungs) and warm ischemia may be at high risk of reperfusion injury after transplantation. Such lungs could be pharmacologically reconditioned using ex-vivo lung perfusion (EVLP). Since acute inflammation related to the activation of nuclear factor kappaB (NF-κB) is instrumental in lung reperfusion injury, we hypothesized that DCD lungs m...

Ex vivo lung perfusion (EVLP) is a promising procedure for evaluation, reconditioning, and treatment of marginal lungs before transplantation. Small animal models can contribute to improve clinical development of this technique and represent a substantial platform for bio-molecular investigations. However, to accomplish this purpose, EVLP models must sustain a prolonged reperfusion without phar...

BACKGROUND Donation after circulatory death (DCD) has the potential to significantly alleviate the shortage of transplantable lungs. We report our initial experience with the use of portable ex vivo lung perfusion (EVLP) with the Organ Care System Lung device for evaluation of DCD lungs. METHODS We performed a retrospective review of the DCD lung transplantation (LTx) experience at a single i...

The introduction of ex vivo lung perfusion (EVLP) in the practice of lung transplantation has allowed the reconditioning of the marginal grafts and their conversion into transplantable grafts. In addition, EVLP can provide a platform for the application of various preventive measures to decrease the incidence of post-transplant complications. While the Toronto team targets the attenuation of th...

OBJECTIVES Ex vivo lung perfusion (EVLP) is a novel technique used to evaluate and recondition marginal or rejected grafts. Primary graft dysfunction (PGD) is a major early complication after lung transplantation (LTx). The use of marginal or initially rejected grafts may increase its incidence and severity. The aim of this study is to evaluate the incidence of PGD after LTx using rejected graf...

In response to the increasing demand for lung transplantation, ex vivo perfusion (EVLP) has extended number of suitable donor lungs by rehabilitating marginal organs. However despite an expanding use in clinical practice, responses different cell types EVLP are not known. order advance our mechanistic understanding and establish a refine tool improvement EVLP, we conducted pioneer study involvi...

PurposeLung weight gain during ex vivo lung perfusion (EVLP) can reduce transplant suitability. Therefore, real-time measurement enhance donor evaluation. We sought to 1) develop/validate a EVLP system, and 2) define the time course of change EVLP.MethodsIn our clinical acellular from 6/2019-9/2020, was measured in 36 cases. Weights were recorded with scale under organ chamber, back table stati...

PurposeAlthough organ donors have high prevalence of pulmonary embolism (PE), the diagnosis PE in donor lungs can be challenging and a detrimental effect on lung transplantion (LTx) outcomes. The aim this study was to investigate feasibility novel technique using ex-vivo artery (EXPLORE) angioscopy for treatment post-procurement.MethodsAn adult fiber optic bronchoscope utilized EXPLORE (off-lab...