In vitro cormogenesis is a potential tool for improving saffron production under controlled conditions. this study, the effects of explant type, culture and medium supplements on daughter corm formation in were assessed. Saffron flowers emerged 30 days after culture, sizes in-vitro- ex-vitro-produced stigmas similar. life cycle was completed 10 14 weeks respectively. Using intact corms more eff...

Oligodendrocytes develop from a subpopulation of precursor cells within the ventral ventricular zone of the spinal cord. The molecular cues that direct this spatially and temporally restricted event seem to originate in part from structures ventral to and within the spinal cord. Here, we present evidence that the family of ligands termed neuregulins are necessary for the normal generation of mo...

In an effort to elaborate the elements of actinidia micropropagation methodology, 2 varieties male form
 (Kamandor, Pryvabny) and female form (Sentyabrskaya, Prevoshodnaya) were selected. The shoot tips forms
 buds lignified shoots forms used as initial explants. regenerative ability explants at stage in vitro culture induction was defined by plant genotype type explant selected: expl...

Cryopreservation features of Lithuanian garlic (Allium sativum L.) cultivars ‘Žiemiai’ and ‘Dangiai’ were investigated. The survival rate state explants, depending on the type explant, dehydration conditions evaluated in experiment. Explants isolated from cloves bulbils dehydrated with a plant vitrification solution 3 (PVS 3) containing 50% w/v glycerol sucrose liquid MS medium. Three different...

BACKGROUND Here we investigate the effect of millicurrent treatment on human chondrocytes cultivated in a collagen gel matrix and on human osteochondral explants. METHODS Human chondrocytes from osteoarthritic knee joints were enzymatically released and transferred into a collagen type-I gel. Osteochondral explants and cell-seeded gel samples were cultivated in-vitro for three weeks. Samples ...

Introduction: Longitudinal bone growth is a complex process involving temporo-spatial progression of chondrocytes throughout the reserve zone, the proliferative zone and the hypertrophic zone of the growth-plate [1]. The growth rate of a bone is a complex interplay between: 1) cell replication in the proliferative zone; 2) cell enlargement in the hypertrophic zone; and 3) matrix synthesis as we...

Many factors may influence the efficiency of somatic embryogenesis. This capability may be differ with regard to media compositions, genotype, tissue, organ ontology and the stage of differentiation. The effects of five salicylic acid concentrations (0, 25, 50, 75 and 100 mM) on different stages of carrot somatic embryogenesis were studied using petiole and root secondary phloem explants as sta...

Cell transformation of Arabidopsis thaliana (L.) Heynh. with Agrobacterium tumefaciens (LBA4404 strain) was performed in two ways: in vitro by the transformation of root and stem explants and inplanta by the transformation of whole plants in the green­ house. Agrobacterium contained the binary plasmid pBI121 or its constructs with the fragments of carrot DC-8 promoter. The results show that tra...

We identified hyaluronan synthase-2 (Has2) as a likely source of hyaluronan (HA) during embryonic development, and we used gene targeting to study its function in vivo. Has2(-/-) embryos lack HA, exhibit severe cardiac and vascular abnormalities, and die during midgestation (E9.5-10). Heart explants from Has2(-/-) embryos lack the characteristic transformation of cardiac endothelial cells into ...