The phospholipid distribution and the fatty acid composition of the individual phospholipids were determined for the nucleus and the bileaflet nuclear membrane. The phospholipid to protein ratio (milligram per mg) for the nuclear membrane was 0.45 and lipid phosphorus comprised 65% of the total membrane phosphorus. No significant differences were noted in the phospholipid composition of the nuc...

The phospholipid distribution and the fatty acid composition of the individual phospholipids were determined for the nucleus and the bileaflet nuclear membrane. The phospholipid to protein ratio (milligram per mg) for the nuclear membrane was 0.45 and lipid phosphorus comprised 65% of the total membrane phosphorus. No significant differences were noted in the phospholipid composition of the nuc...

The phospholipid distribution and the fatty acid composition of the individual phospholipids were determined for the nucleus and the bileaflet nuclear membrane. The phospholipid to protein ratio (milligram per mg) for the nuclear membrane was 0.45 and lipid phosphorus comprised 65% of the total membrane phosphorus. No significant differences were noted in the phospholipid composition of the nuc...

The synthesis of racemic 1,2-dioleoyl and 1 -oleoyl-2-stearoyl glycerol-3-phosphoryl serine was achieved by the phosphorylation of unsaturated diglycerides with phosphorus oxychloride, and combination with the phthalimidomethyl ester of carbo-t-butoxy-DL-serine. The synthetic phosphatidyl serines were tested in blood clotting systems in vitro. They have activity qualitatively similar to that of...

Introduction Trauma patients are susceptible to coagulopathy and dysfunctional immune responses. Mesenchymal stromal cells (MSCs) at the forefront of cellular therapy revolution with profound immunomodulatory, regenerative, therapeutic potential. Routine assays assess immunomodulation activity examine MSC effects on proliferation peripheral blood mononuclear (PBMCs) take 3–7 days. Assays that c...

The specificity of the phospholipid cofactor requirement of rat brain protein kinase C was investigated using Triton X-100 mixed micellar methods. Sixteen analogues of phosphatidylserine were prepared and tested for their ability to support protein kinase C activity, [3H]phorbol 12,13-dibutyrate binding, and protein kinase C binding to mixed micelles. Phosphatidylserinol, -L-serine methyl ester...

The influence of diabetes and insulin treatment on the phospholipid content of R3230AC mammary tumors, a hormonally responsive neoplasm, was studied. Diabetes was induced by administration of streptozotocin 3 days prior to tumor implantation. Protamine zinc insulin, 3 IU/rat twice daily, was administered to tumor-bearing rats for 3 days. Enzymatically dissociated tumor cells from diabetic anima...