The metabolism of [3H]benzo(a)pyrene [B(a)P] by cultures of primary mouse epidermal cells and untransformed and transformed epidermal cell lines was investigated. All three cell types effectively metabolized [3H]B(a)P. The major organic solvent-extractable metabolites found intracellularly in primary cultures were trans-7,8-dihydro-7,8-dihydroxybenzo(a)pyrene and 3-hydroxybenzo(a)pyrene, althou...

BACKGROUND Aflatoxin B1 (AFB1), produced by Aspergillus flavus, is one of the most life threatening food contaminants causing significant economic losses worldwide. Biological AFB1 degradation by microorganisms, or preferably microbial enzymes, is considered as one of the most promising approaches. OBJECTIVES The current work aimed to study the AFB1-degrading metabolites, produced by Phoma gl...

Choline and choline metabolites are essential for all cellular functions. They have also been reported to be crucial neural development. In this work, we studied the functional characteristics of uptake system in human stem cells (hNSCs). Additionally, investigated effect extracellular inhibition on activities hNSCs. We found that mRNAs proteins transporter-like protein 1 (CTL1) CTL2 were expre...

Materials and methods Experimental set-up CHO-K1 cells were cultivated in protein free TC-42 medium (TeutoCell, Bielefeld, Germany) in 250 ml baffled shake flasks. For the non-stationary experiment the cultures were inoculated at a start cell density of 2 × 10 cells/ml in a start volume of 120 ml. Four parallel cultivations were performed, two with 100% [U-C6] glucose and two with 100% [U-C5]gl...

Vector borne diseases are affecting millions of people annually. Entomopathogenic fungi toxicity on malaria vectors increase mortality rates, and are less effective on nontarget organisms, thus representing a control measure that could be used in integrated programmes. Before metabolites of entomopathogenic fungi can be integrated into control programmes, an effective delivery system must be de...

The acute effect of 3,4-methylenedioxyamphetamine (MDA) and three structural analogues on the extracellular concentrations of dopamine (DA) and its major metabolites, dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), in the striatum was studied using in vivo microdialysis in awake, freely moving rats. MDA significantly (P less than 0.001) increased and decreased the extracellular ...

According to studies, the microbiome may contribute emergence and spread of breast cancer. E. coli is one Enterobacteriaceae family recently found be present as part tissue microbiota. In this study, we focused on effect secretome free cells MCF-7 metabolism. Liquid chromatography–mass spectrometry (LC-MS) metabolomics was used study its role in intra- extracellular metabolites. A comparison ma...