BACKGROUND & OBJECTIVE The conventional culture technique for diagnosis of extrapulmonary tuberculosis is time consuming. In order to find a sensitive and rapid technique nested polymerase chain reaction (nPCR) targeting the conserved MPB 64 gene of Mycobacterium tuberculosis was evaluated for detection of M. tuberculosis DNA directly from clinical specimens of extrapulmonary origin. METHODS ...

Each year, there are more than eight million new cases of tuberculosis and 1.3 million deaths. There is a renewed interest in extrapulmonary forms of tuberculosis as its relative frequency increases. Among extrapulmonary organs, pleura and lymph nodes are the most common. Their diagnosis is often difficult and is based on clinical, radiological, bacteriological and histological findings. Extrap...

316 Tuberculosis can involve any organ system in the body. While pulmonary tuberculosis is the most common presentation, extrapulmonary tuberculosis (EPTB) is also an important clinical problem. The term EPTB has been used to describe isolated occurrence of of tuberculosis at body sites other than the lung. However, when an extrapulmonary focus is evident in a patient with pulmonary tuberculosi...

Objective: To compare the performance of nested polymerase chain reaction (NPCR) with that of cultures in the detection of the Mycobacterium tuberculosis complex in pulmonary and extrapulmonary specimens. Methods: We analyzed 20 and 78 pulmonary and extrapulmonary specimens, respectively, of 67 hospitalized patients suspected of having tuberculosis. An automated microbial system was used for th...

Although Tuberculosis (TB) most commonly affects the lungs, any organ or tissue can be involved. In countries with comprehensive diagnostic and reporting systems, extrapulmonary tuberculosis (EPTB) accounts for 20-25% of reported cases. Globally, extrapulmonary cases (without concurrent pulmonary involvement) comprised 14% of notified cases (new and relapse) in 2007. The most common sites of ex...

Tuberculosis (TB) is a re-emerging disease and an important cause of mortality. It manifests in two forms: pulmonary, which affects only the lungs, extrapulmonary, defined as bacteriologically confirmed or clinically diagnosed infection by Mycobacterium tuberculosis that involves organs other than being pleural one most common. Extrapulmonary usually result hematogenous dissemination pulmonary ...

Although the respiratory tract is the major site for tuberculous lesions, all organs can be affected. The authors review the clinical files of 25 patients admitted to our Department with lung and/or pleural tuberculous disease, confirmed by bacteriology or histopathology, who simultaneously suffered from tuberculosis in other organs. We included 20 men and 5 women, aged from 10 to 74 years (mea...

INTRODUCTION Tuberculosis remains a major public health problem in developing countries. Diagnosing extrapulmonary tuberculosis can be difficult, as it requires a higher index of suspicion than primary tuberculosis. Extrapulmonary tuberculosis may mimic malignancies and many other diseases, so it should be included in the differential diagnosis. Here, we present a case of extrapulmonary tubercu...

Introduction: In HIV-associated tuberculosis cases, identifying nontuberculous Mycobacteria (NTM) from clinical samples has become essential regarding patients’ treatment and prognosis. This study aims to determine the prevalence of different Mycobacteria species from immunocompromised and immunocompetent patients with suspected tuberculosis attending a tertiary care hospital in Kolkata, India....

AIMS Use of the polymerase chain reaction for the detection of Mycobacterium tuberculosis (TB PCR) as a basis for making clinical decisions on the initiation of antituberculosis treatment was studied. METHODS A retrospective study involving a cohort of 155 patients being investigated for tuberculosis in an infectious disease consultation service was undertaken. TB PCR was performed on pulmona...