The abbreviations used are: PPAR, peroxisome proliferator-activated receptor; PPRE, peroxisome proliferator response element(s); AOX, straight chain fatty acyl-CoA oxidase; L-PBE, peroxisomal enoylCoA hydratase/3-hydroxyacyl-CoA dehydrogenase bifunctional protein; PTL, peroxisomal 3-ketoacylCoA thiolase; G-6-P, glucose-6-phosphatase; C/EBPα, CCAAT enhancer-binding protein α; SREBP1, sterol regu...

background: analysis of human cancer serum has revealed the presence of high amounts of hyaluronan (hyaluronic acid, ha) when compared to human normal serum, it is well documented that ha and its receptors, known as hyaladherins (habps) are involved in matrix regulation, cell proliferation, migration and malignant tumour progression. these hyaladherins not only interact with hyaluronan at the m...

OBJECTIVES Necrotizing enterocolitis is a severe multifactorial intestinal disorder that primarily affects preterm newborns, causing 20-40% mortality and morbidity. Intestinal fatty acid-binding protein has been reported to be a biomarker for the detection of intestinal injuries. Our aim was to assess intestinal tissue injury and the molecular expression of intestinal fatty acid-binding protein...

Weisiger, Richard A. Saturable stimulation of fatty acid transport through model cytoplasm by soluble binding protein. Am. J. Physiol. 277 (Gastrointest. Liver Physiol. 40): G109–G119, 1999.—To better define the role of soluble binding proteins in the cytoplasmic transport of amphipathic molecules, we measured the diffusional mobility of a fluorescent long-chain fatty acid, 12-N-methyl-(7-nitro...

EgFABP1 is a developmentally regulated intracellular fatty acid binding protein characterized in the larval stage of parasitic platyhelminth Echinococcus granulosus. It is structurally related to the heart group of fatty acid binding proteins (H-FABPs). Binding properties and ligand affinity of recombinant EgFABP1 were determined by fluorescence spectroscopy using cis- and trans-parinaric acid....

The rate of transition from an unstable to a stable complex and the dependence of this on the number of fatty acid ligands present was determined for the binding of warfarin on human serum albumin. When oleate or palmitate was added in amounts up to 2:1 excess to human serum albumin solutions the measured rate constant for the transition (k2) was increased in comparison with fatty acid free alb...

Fatty acid-binding protein from rat liver is shown to bind the fluorescent fatty acid probe dansyl undecanoic acid. Binding is accompanied by a shift in the fluorescence emission maximum from 550 nm to 500 nm and a 60-fold fluorescence enhancement at 500 nm. These spectral properties have allowed the use of this probe to detect and quantify microgram amounts of liver fatty acid-binding protein ...