Therapies that target tumor metabolism represent a new horizon in anticancer therapies. In particular, cancer cells are dependent on the generation of lipids, which are essential for cell membrane synthesis, modification of proteins, and localization of many oncogenic signal transduction enzymes. Because fatty acids are the building blocks of these important lipids, fatty acid synthase (FASN) e...

After permeability barrier perturbation there is an increase in the mRNA levels for key enzymes necessary for lipid synthesis in the epidermis. The mechanism(s) responsible for this regulation is unknown. Sterol regulatory element binding proteins-1a, 1c, and -2 (SREBPs) control the transcription of enzymes required for cholesterol and fatty acid t synthesis in response to modulations of sterol...

s 1. Little, J.L., Fels D.R., Koumenis, C., and Kridel, S.J., (2005) Orlistat induces ER stress in prostate tumor cells. American Association for Cancer Research (AACR). Anaheim, California. 2. Little, J.L., Wheeler, F.B., Fels, D.R., Koumenis, C., and Kridel, S.J. Inhibition of fatty acid synthase induces ER stress-dependent cell death. American Association for Cancer Research (AACR), Washingt...

Fatty acid synthase from the uropygial gland of goose was inhibited by increasing the [NADP]:[NADPH] ratio. NADP inhibition of the overall activity of fatty acid synthase, the ketoreductase, and the enoyl reductase were competitive with respect to NADPH with Ki values of 6 p ~ , 40 p ~ , and 260 pM, respectively. The other component activities of fatty acid synthase were not affected by NADP wi...

One of the key limiting factors in the treatment of advanced stage human epithelial malignancies is the lack of selective molecular targets for antineoplastic therapy. A substantial subset of human ovarian, endometrial, breast, colorectal, and prostatic cancers exhibit increased endogenous fatty acid biosynthesis and overexpress certain enzymes in the pathway. Cell lines derived from these tumo...

Cerulenin, [(2S,3R)-2,3-epoxy-4-oxo-7,10-dodecadienoylamide], a mycotoxin produced by Cephalosporium caerulens, irreversibly inactivated 6-methylsalicylic acid synthase from Penicillium patulum. A combination of radiolabelling studies with [3H]cerulenin, proteolytic and chemical digestion and N-terminal sequencing of labelled peptides indicated that the site of cerulenin modification is the hig...

One of the key limiting factors in the treatment of advanced stage human epithelial malignancies is the lack of new, selective molecular targets for antineoplastic therapy. A substantial subset of human breast, ovarian, endometrial, colorectal, and prostatic cancers express elevated levels of fatty acid synthase, the major enzyme required for endogenous fatty acid biosynthesis, and carcinoma li...

FAS (Fatty acid synthase) an enzyme catalysing synthesis of fatty acid. The protein contains two subunits,namely A & B. Fatty acid synthase protein plays a key role in fat deposition in chicken as it was found that the protein is directly correlated with fat content of chicken. Administration of glucocorticoids changes the expression of FAS protein.3D structure of FAS protein was not available ...

In order to identify regulatory steps in fatty acid biosynthesis, the influence of intermediate 3-oxoacyl-(acyl carrier proteins) (3-oxoacyl-ACPs) and end-product acyl-ACPs of the fatty acid synthase reaction on the condensation reaction was investigated in vitro, using total fatty acid synthase preparations and purified 3-oxoacyl-ACP synthases (KASs; EC 2.3.1.41) from Cuphea lanceolata seeds. ...