The indirect fluorescent-antibody (IFA) method for diagnosis of toxoplasmosis is widely used and is considered to be as specific as the Sabin-Feldman dye test. After observing a patient with systemic lupus erythematosus (SLE) who had a positive toxoplasma IFA test but a negative dye test, we studied sera with high titers of antinuclear antibodies from 16 SLE patients and from 2 with rheumatoid ...

An immunoglobulin M (IgM)-immunosorbent agglutination assay (IgM-IS-AGA) was negative in all sera from individuals negative in the Sabin-Feldman dye test, in sera from individuals with chronic Toxoplasma infection, and in cord blood samples from uninfected infants. In contrast, all sera that were obtained from individuals with a recent history of acute Toxoplasma infection and from infants with...

An epidemic of cases of toxoplasmic encephalitis is occurring in patients with acquired immune deficiency syndrome (AIDS). Serological or histopathologic studies were performed on 70 cases with AIDS and toxoplasmic encephalitis. In many cases conventional stains of brain-tissue specimens failed to disclose Toxoplasma organisms; all were positive when stained by the peroxidase-antiperoxidase tec...

Infection with Toxoplasma gondii is often asymptomatic and, when acquired during pregnancy, may lead to connatal toxoplasmosis in the offspring. The newly introduced Vitros anti-Toxoplasma immunoglobulin G (IgG) and IgM assays, designed for the Vitros ECiQ immunodiagnostic system, a fully automated system based on chemiluminescence, were evaluated as a screening method for the serological detec...

Serological methods for the diagnosis of toxo-plasmosis have received considerable attention since the first authentic report of a fatal infection in a human infant (Wolf, Cowen, and Paige, 1939). The complement fixation reaction (Warren and Sabin, 1942) and the dye test (Sabin and Feldman, 1948) are now recognized as the two best methods of detecting the disease. Serological studies in sarcosp...

With the ever-increasing interest being taken in toxoplasmosis more and more requests are reaching laboratories for help in its diagnosis. Prominent among the diagnostic tests employed is the cytoplasm-modifying-antibody (dye) test introduced by Sabin and Feldman (1948). Briefly, this consists in mixing varying dilutions of the patient's serum with peritoneal exudate rich in Toxoplasma from an ...

The dye test for toxoplasmosis (Sabin and Feldman, 1948) depends upon the fact that toxoplasma organisms lose their affinity for methylene blue when they are exposed to specific antibody plus a heat-labile " accessory factor" at present available only from certain human sera. Accessory factor has been shown to differ from complement in several respects. Sera containing antibodies which produce ...

The dye test for toxoplasmosis (Sabin and Feldman, 1948) depends upon the fact that toxoplasma organisms lose their affinity for methylene blue when they are exposed to specific antibody plus a heat-labile " accessory factor" at present available only from certain human sera. Accessory factor has been shown to differ from complement in several respects. Sera containing antibodies which produce ...

The dye test for toxoplasmosis (Sabin and Feldman, 1948) depends upon the fact that toxoplasma organisms lose their affinity for methylene blue when they are exposed to specific antibody plus a heat-labile " accessory factor" at present available only from certain human sera. Accessory factor has been shown to differ from complement in several respects. Sera containing antibodies which produce ...