samples from the kidney of 100 diseased fish with signs of haemorrhagic septicemia and 50 samples from outlet water of fish farms were taken aseptically and cultured. in the laboratory, 75 colonies of gram-negative bacteria were biochemically diagnosed as motile aeromonads in our bacteriology laboratory unit using api 20e rapid identification system. the genotype identification using specific p...

A highly pathogenic isolate of Aeromonas hydrophila, isolate No1, was identified through evaluation of the Crude extra-cellular product and estimation of LD50. Three types of formalized whole culture Aeromonas hydrophila vaccine (FWC) were prepared, FWC vaccine alone, FWC vaccine mixed with Freund's complete adjuvant (FCA), and FWC vaccine mixed with Freund's incomplete adjuvant (FIA), tested f...

carnation etched ring virus (cerv) is a member of the caulimovirus genus of the caulimoviridae. the virus is the second most important virus of carnation around the world and the only dna virus among infecting viruses of carnation. the virus infected plants were collected from greenhouse in chenaran and two fragments of ~1500 bp and ~1000 bp respectively for coat protein and movement protein we...

edwardsiella tarda is a versatile pathogen that could survive in different environmental conditions and infect economically important fish species. the whole cell antigenic proteins were extracted from e. tarda and assessed via western blot using anti e. trada rohu and anti e. trada rabbit serum. two strong reacted proteins with antibodies were recovered viz 61 kda and 47 kda from sds page gels...

The by-product from tuna processing is a potential source of edible protein. Therefore, it is very important to extract protein from such raw materials for human food. In this study the optimum pH for protein extraction from tuna by-products was optimized by using isoelectric solubilization and precipitation processes. The Response Surface Methodology (RSM) and the single factor model were used...

Vibrio anguillarum causes a fatal hemorrhagic septicemia in marine fish that leads to great economical losses in aquaculture world-wide. Vibrio anguillarum strain NB10 serotype O1 is a Gram-negative, motile, curved rod-shaped bacterium, isolated from a diseased fish on the Swedish coast of the Gulf of Bothnia, and is slightly halophilic. Strain NB10 is a virulent isolate that readily colonizes ...