X35-APS-This substrate was synthesized from S%ulfur trioxide-pyridine complex and AMP by a method similar to that of Baddiley et al. (13) except for simplifications and modifications necessary for introduction of label. An alternat,ive method has been described by Reichard and Ringertz (14). Approximately 3.7 me of S35-HzS04 was dried at 80” in a reaction tube fitted with a ball joint and side ...

This study is pertaining to the production of penicillin G acylase (PGA) by Bacillus subtilis 8105MU331 in which PGA gene, under the control of thermal-induced promoter, was integrated. The key process parameters including induced-temperature, induced-time, and culture temperature were optimized in flask culture. A three-stage cultivation process was developed for PGA production with the expres...

1T HE DUMBELL-DIFFUSION CELL described by Nobel (1) is made from a ball and socket joint, each end of which is formed into a bell-shaped flask. This cell is applicable to rapid testing of blood for the presence or absence of various toxic constituents. it allows diffusion, absorption, and i(lentiflcation reactions to proceed concurrently under a variety of conditions (Table 1). [sing this appar...

in recent years, biosurfactants due to wide applications in chemical, petroleum, food andpharmaceutical industries, have been widely considered by researchers. biosurfactants are produced by aseries of microorganisms, so it is important to screen culture medium and operating conditions in miniaturized bioreactors prior to scaling up to large bioreactors.in this study, using a kind of miniaturiz...

The purpose of this study was to verify the influence of flask closure methods and post-pressing times on tooth displacement in maxillary dentures. Twenty similar maxillary dentures were made using a heat-polymerized acrylic resin, and randomly assigned to 4 experimental groups (n=5) according to the protocol: 1-conventional flask closure and immediate polymerization; 2- conventional flask clos...