The present review article presents and discusses automated sequential injection (SI) based methods for the determination of pharmaceutically and biologically important thiols, namely cysteine, N-acetylcysteine, penicillamine, glutathione and captopril. The review intends to cover a range of ten years of published research on this topic (2000 – 2009). The methods are classified according to the...

cells, proteins, and small molecules from the alveolar space of the lower respiratory tract since the first report of Reynolds and Newball over three decades ago. The analysis of these components is of wide diagnostic and investigative value. In bronchoalveolar lavage, sterile saline is administered into the bronchoalveolar space and aspirated by syringe. Because recovery of the components in b...

Since the observation of Desha (1) that the fluorescence exhibited by solutions of quinine and aniline erythrosine when irradiated by ultraviolet light could be utilized for their quantitative determination, useful fluorimetric methods have been devised for a number of biological constituents (2). The extreme sensitivity of fluorimetric assay offers a distinct advantage over other chemical and ...

An alternative method for the determination of trace levels of fluoride in drinking and sea-water samples is presented. It is based on the formation of the aluminium monofluoride complex in excess of Al3+ and separation of the two species formed (AlF2+ and Al3+) in a small (5 cm long, CG2) ion exchange guard column. The final determination is accomplished by both ICP-MS specific detection and p...

A precise and reproducible method is described for the ultramicro fluorimetric determination of calcium in plasma, based upon the formation of a fluorescent complex between calcium and calcein at a strongly alkaline pH. In a group of normal subjects, the mean concentration of plasma calcium was 9.66 mg/100 ml with a normal range of 8.9 to 10.4 mg/100 ml.

Di-2-pyridyl ketone 2-furoylhydrazone (DPFH) has been examined as a sensitive spectrofluorimetric reagent for the determination of gallium. 4 rapid procedure for the fluorimetric determination of th i s ion at the 2-800 ng ml-1 level at pH 3.S4.1 (Aex 395 nm, he, 473 nm) has been established. The detection limit is 2 ng ml-1 and the relative standard deviation is 2.4% (40 p.p.b. of gallium). In...