Tissue fixation in phosphate buffered formalin (PBF) remains the standard procedure in histopathology, since it results in an optimal structural, antigenic and molecular preservation that justifies the pivotal role presently played by diagnoses on PBF-fixed tissues in precision medicine. However, toxicity of formaldehyde causes an environmental concern and may demand substitution of this reagen...

TESTING OF SACCHAROMYCES CEREVISIAE MORPHOLOGICAL FIXATIVES AND FIXED SAMPLES STORED AT AMBIENT TEMPERATURE Diana Ly, Diane Yu, Beverly Girten and Jacob Cohen Education Associates Program (University of San Francisco), Lockheed Martin Space Operations, NASA Ames Research Center, Science and Technology Corporation, Moffett Field, CA EMMYS-1, Effects of Microgravity on Model Yeast Specimen, is a ...

We show that two distinct distributions of F-actin are present in the hyphal apex of the oomycete Achlya bisexualis, that have been chemically fixed with a combination of methylglyoxal and formaldehyde and stained with Alexa phalloidin. In approximately one half of the hyphae examined, an F-actin depleted zone within the apical F-actin cap was observed. The remaining hyphae had a continuous api...

The effects of formaldehyde, acetaldehyde, and glutaraldehyde on human red blood cells were investigated. It was found that (a) The surface negative charge of the erythrocytes at pH 7 was increased 10% by glutaraldehyde, but not by the other two aldehydes. (b) The effect of incomplete fixation of the red blood cells was demonstrated by hemoglobin leakage studies The leakage of hemoglobin subseq...

Formalin is widely used in industry and in medicine (as tissue fixative and disinfectant).It contains reactive molecules which have been known for its cytotoxic effects. To evaluate the effect of formalin exposure on the testicular tissue and sperm parameter from neonatal period through physical and sexual maturity, 28 male Wister rats were assigned into two equal test and control groups. The t...

1. The Kultschitzky cells of the alimentary canal of man and the guinea-pig were studied with the light-microscope, buffered osmium tetroxide being used as fixative. 2. The Kultschitzky cells of the guinea-pig were shown by the electron-microscope to contain spheroidal granules having a maximum diameter of o-3/x. These granules are considerably darkened by fixation for only 4 hours in 1 % osmiu...

In situ hybridization (ISH) is widely used to detect DNA and RNA sequences within the cell and tissue sections. The important step in performing this technique is tissue fixation. We investigated the influence of the pH of the fixative on the outcome of ISH. Our studies indicate that alkaline formaldehyde dramatically increases the ISH signal with RNA probes. The increase in signal was observed...

As part of an overall evaluation of possible substitutes for the pulpotomy agent formocresol, this study was initiated to compare the antigenicity of the reaction products of protein with formaldehyde, glutaraldehyde, or dimethylsuberimidate (DMS). Rabbits were injected with rabbit serum albumin (RSA) which had been treated with one of the following solutions: phosphate-buffered saline, 2% glut...