نتایج جستجو برای: frozen ova
تعداد نتایج: 33899 فیلتر نتایج به سال:
the immune responses of mice immunized with ovalbumin (ova) together with killed l. major (klm) promastigotes as adjuvant were studied. three doses (5 × 107, 1 × 108 and 2 × 108) of klm combined with ova (100 mg) were injected into the groups of c57bl/6 mice. bcg and complete freund’s adjuvant (cfa) were used as control adjuvants. lymphocyte proliferation and antibody titers were determined, an...
Murine dendritic cells (DCs) are widely used for experimental vaccinations in mouse models. A high-yield method for freezing and thawing batches of these cells, if compatible with retention of cell immunophenotype, would reduce the time required for repeated preparations from DC precursors in bone marrow (BM), as well as variability among lots. Following depletion of specific lineages, murine b...
Objective(s): Tanshinone IIA (T. IIA), one of the most pharmacologically active components extracted from Salviae miltiorrhiza, has anti-inflammatory and antioxidant features. The aim of the present study is to investigate the benefit of T. IIA on asthma using a murine model of asthma induced by ovalbumin (OVA). Materials and Methods: Male BALB/c mice were used in the present study. The mice we...
Purpose: To evaluate the protective effect of cavidine against asthma in neonatal mice.
 Methods: Neonatal mice were treated with at doses 5 and 10 mg/kg, po, 2 h prior to induction ovalbumin (OVA) on 1st 14th days treatment protocol. The anti-asthma activity was evaluated by determining number inflammatory cells cytokine levels broncho-alveolar lavage fluid (BALF) OVA-specific IgE TGF-β 1...
Background and objectives Wheat gluten (WG) often co-exists with egg white (EW) whey proteins in foods. This study investigated the impact of WG on denaturation hen (S-)ovalbumin [(S-)OVA], lysozyme (LYS), bovine serum albumin (BSA). Findings caused peak temperature LYS BSA to increase that (S-)OVA decrease. It accelerated decelerated OVA, respectively, their lowered activation energy. In contr...
Allergic airway inflammation is attenuated by oral tolerization (oral exposure to allergen, followed by conventional sensitization and challenge with homologous antigen), which decreases airway allergen challenge-induced eosinophilic infiltration of the lungs and bone marrow eosinophilia. We examined its effects on bone marrow eosinophil and neutrophil production. Mice of wild type (BP-2, BALB/...
Background: The events that trigger type 2 immune responses are not well characterized. Given the central role of sweat in maintaining skin hydration, sweating disturbance at points allergen entry would predispose to food sensitization. A previous study demonstrated epicutaneously applied hapten more readily penetrates stratum corneum with a lower water content than higher water. Thus, is likel...
BACKGROUND Modified vaccinia virus Ankara (MVA)-encoding antigens are considered as safe vaccine candidates for various infectious diseases in humans. Here, we investigated the immune-modulating properties of MVA-encoding ovalbumin (MVA-OVA) on the allergen-specific immune response. METHODS The immune-modulating properties of MVA-OVA were investigated using GM-CSF-differentiated BMDCs from C5...
Objective(s): Human and animal studies have shown a close relationship between obesity and asthma severity. Here, we examined the effects of diet-induced obesity (DIO) on the expression levels of IL-1β, IRAK-1 and TRAF-6 mRNA in male Wistar rats tracheal after sensitization with ovalbumin (OVA). Materials and Methods:Twenty male Wistar rats divided to four groups, included, control group with ...
ContextSaikosaponin D (SSD) is a commonly prescribed agent against inflammatory diseases in Asian countries. However, the anti-allergic effect of SSD allergic rhinitis (AR) model not well known.ObjectiveWe investigated and anti-inflammatory effects on ovalbumin (OVA)-induced AR model.Materials methodBALB/c mice were divided into control, OVA, OVA + SSD, dexamethasone (Dex) groups. was establish...
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